EPN1 antibody

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Rabbit anti EPN1

Rabbit anti human EPN1 antibody recognizes Epsin-1, also known as EH domain-binding mitotic phosphoprotein or EPS-15-interacting protein 1. This antibody has been extensively validated for western blotting using whole cell lysates.

EPN1 Antibody with Control Lysate

Rabbit anti human EPN1 antibody recognizes Epsin-1, also known as EH domain-binding mitotic phosphoprotein or EPS-15-interacting protein 1. This antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00306 WB 100 µl
VPA00306KT WB 2 Westerns
With Lysate 400µg
Rabbit anti Human EPN1 antibody recognizes EPN1, also known as EH domain-binding mitotic phosphoprotein, Epsin 1 or EPS-15-interacting protein 1.

The protein encoded by the EPN1 gene binds clathrin and is involved in the endocytosis of clathrin-coated vesicles. Three transcript variants encoding different isoforms have been found for EPN1 (provided by RefSeq, Nov 2011).

Rabbit anti Human EPN1 antibody detects a band of 90 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography
Preparation
20μl Rabbit polyclonal antibody purified by affinity chromatography
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
KLH-conjugated synthetic peptide corresponding to aa 203-232 of human EPN1

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch
Shelf Life
As supplied, 12 months from date of despatch

More Information

UniProt
Q9Y6I3 Related reagents
Entrez Gene
EPN1 Related reagents
GO Terms
GO:0005886 plasma membrane
GO:0005634 nucleus
GO:0005737 cytoplasm
GO:0005905 coated pit
GO:0008289 lipid binding
GO:0006897 endocytosis
GO:0007173 epidermal growth factor receptor signaling pathway
GO:0042059 negative regulation of epidermal growth factor receptor signaling pathway
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of EPN1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Western Blotting
Anti EPN1 detects a band of approximately 90 kDa in HEK293 cell lysate
Western Blotting
Anti EPN1 detects a band of approximately 90 kDa in HEK293 cell lysate
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB