Endophilin B1 antibody

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Rabbit anti Endophilin B1

Rabbit anti human endophilin B1 antibody recognizes endophilin-B1, also known as Bax-interacting factor 1, BIF1, SH3 domain-containing GRB2-like protein B1, or SH3-containing protein SH3GLB1. This antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00401 WB 100 µl
Rabbit anti Human endophilin B1 antibody recognizes endophilin B1, also known as Bax-interacting factor 1 or PPP1R70.

Endophilin B1 is a SRC homology 3 domain-containing protein. This protein interacts with the proapoptotic member of the Bcl-2 family, Bcl-2-associated X protein (Bax) and may be involved in regulating apoptotic signaling pathways. It may also be involved in maintaining mitochondrial morphology. Alternate splicing results in multiple transcript variants (provided by RefSeq, Sep 2011).

Rabbit anti Human Endophilin B1 antibody detects a band of 42 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti endophilin B1 detects a band of approximately 42 kDa in C2C12 cell lysate
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
KLH-conjugated synthetic peptide corresponding to aa 59-86 of human endophilin B1
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles
Shelf Life
As supplied, 12 months from date of despatch

More Information

UniProt
Q9Y371 Related reagents
Entrez Gene
SH3GLB1 Related reagents
GO Terms
GO:0000139 Golgi membrane
GO:0006915 apoptosis
GO:0007165 signal transduction
GO:0005741 mitochondrial outer membrane
GO:0008289 lipid binding
GO:0006916 anti-apoptosis
GO:0008093 cytoskeletal adaptor activity
GO:0017124 SH3 domain binding
GO:0042803 protein homodimerization activity
GO:0046847 filopodium assembly
Acknowledgements
PrecisionAbâ„¢ is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of Endophilin B1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB

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