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ENDOPHILIN-A1 antibody

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Anti ENDOPHILIN-A1 Antibody gallery image 1
Western blot analysis of HeLa, BT474 and 293T cell lysates using Rabbit anti Endophilin-A1 antibody at a 1/1000 dilution. 3% non-fat dry milk was used for blocking

Rabbit anti ENDOPHILIN-A1

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP2723
Datasheet
WB
MSDS
50 µl loader

Rabbit anti endophilin-A1 antibody recognizes endophilin-A1, also known as SH3 domain protein 2A, SH3 domain protein 2A and EEN-B1. Endophilins belong to the family of Bin/Amphiphysin/Rvs (BAR) proteins and are composed of an N-terminal BAR domain and a C-terminal SH3 domain (Safari and Suetsugu 2012). Endophilin-A1 is essential for synaptic vesicle endocytosis (Llobet et al. 2011). Its interaction with ataxin-2 and huntingtin is important for plastin-associated cellular pathways (Ralser et al. 2005). Endophilin-A1 is also involved in regulation of blood-brain barrier permeability through the EGFR-JNK signaling pathway (Chen et al. 2015).

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to endophilin-A1 was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide
50% Glycerol
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human endophilin-A1

Storage Information

Storage
Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
Q99962
Entrez Gene
SH3GL2
GO Terms
GO:0005886 plasma membrane
GO:0000139 Golgi membrane
GO:0005829 cytosol
GO:0008289 lipid binding
GO:0006892 post-Golgi vesicle-mediated transport
GO:0006897 endocytosis
GO:0007173 epidermal growth factor receptor signaling pathway
GO:0007411 axon guidance
GO:0007417 central nervous system development
GO:0042802 identical protein binding
GO:0042059 negative regulation of epidermal growth factor receptor signaling pathway
GO:0048011 nerve growth factor receptor signaling pathway
Regulatory
For research purposes only

Applications of ENDOPHILIN-A1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/500 1/2000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
Rabbit anti endophilin-A1 antibody detects a band of approximately 42 kDa in various cell lysates under reducing conditions
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
TidyBlot Western Blot Detection Reagent:HRP STAR209P WB* 0.5 ml
Request a different product with the ENDOPHILIN-A1 specificity