Rabbit anti endophilin-A1 antibody
recognizes endophilin-A1, also known as SH3 domain protein 2A, SH3 domain protein 2A and EEN-B1. Endophilins belong to the family of Bin/Amphiphysin/Rvs (BAR) proteins and are composed of an N-terminal BAR domain and a C-terminal SH3 domain (Safari and Suetsugu 2012
). Endophilin-A1 is essential for synaptic vesicle endocytosis (Llobet et al. 2011
). Its interaction with ataxin-2 and huntingtin is important for plastin-associated cellular pathways (Ralser et al. 2005
). Endophilin-A1 is also involved in regulation of blood-brain barrier permeability through the EGFR-JNK signaling pathway (Chen et al. 2015
- Target Species
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to endophilin-A1 was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide
- Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human endophilin-A1
- Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
post-Golgi vesicle-mediated transport
epidermal growth factor receptor signaling pathway
central nervous system development
identical protein binding
negative regulation of epidermal growth factor receptor signaling pathway
nerve growth factor receptor signaling pathway
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of ENDOPHILIN-A1 antibody
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- Rabbit anti endophilin-A1 antibody detects a band of approximately 42 kDa in various cell lysates under reducing conditions
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
How to Use the Spectraviewer?
Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
Select the lasers and filters you wish to include
Select combined or multi-laser view to visualize the spectra