Rabbit anti endophilin-A1 antibody
recognizes endophilin-A1, also known as SH3 domain protein 2A, SH3 domain protein 2A and EEN-B1. Endophilins belong to the family of Bin/Amphiphysin/Rvs (BAR) proteins and are composed of an N-terminal BAR domain and a C-terminal SH3 domain (Safari and Suetsugu 2012
). Endophilin-A1 is essential for synaptic vesicle endocytosis (Llobet et al. 2011
). Its interaction with ataxin-2 and huntingtin is important for plastin-associated cellular pathways (Ralser et al. 2005
). Endophilin-A1 is also involved in regulation of blood-brain barrier permeability through the EGFR-JNK signaling pathway (Chen et al. 2015
- Target Species
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to endophilin-A1 was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide
- Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human endophilin-A1
- Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
post-Golgi vesicle-mediated transport
epidermal growth factor receptor signaling pathway
central nervous system development
identical protein binding
negative regulation of epidermal growth factor receptor signaling pathway
nerve growth factor receptor signaling pathway
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of ENDOPHILIN-A1 antibody
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- Rabbit anti endophilin-A1 antibody detects a band of approximately 42 kDa in various cell lysates under reducing conditions
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Secondary Antibodies Available