ENDOPHILIN-A1 antibody
Rabbit anti endophilin-A1 antibody recognizes endophilin-A1, also known as SH3 domain protein 2A, SH3 domain protein 2A and EEN-B1. Endophilins belong to the family of Bin/Amphiphysin/Rvs (BAR) proteins and are composed of an N-terminal BAR domain and a C-terminal SH3 domain (Safari and Suetsugu 2012). Endophilin-A1 is essential for synaptic vesicle endocytosis (Llobet et al. 2011). Its interaction with ataxin-2 and huntingtin is important for plastin-associated cellular pathways (Ralser et al. 2005). Endophilin-A1 is also involved in regulation of blood-brain barrier permeability through the EGFR-JNK signaling pathway (Chen et al. 2015).
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to endophilin-A1 was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide
50% Glycerol - Immunogen
- Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human endophilin-A1
Storage Information
- Storage
- Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- Q99962
- Entrez Gene
- SH3GL2
- GO Terms
- GO:0005886 plasma membrane
- GO:0000139 Golgi membrane
- GO:0005829 cytosol
- GO:0008289 lipid binding
- GO:0006892 post-Golgi vesicle-mediated transport
- GO:0006897 endocytosis
- GO:0007173 epidermal growth factor receptor signaling pathway
- GO:0007411 axon guidance
- GO:0007417 central nervous system development
- GO:0042802 identical protein binding
- GO:0042059 negative regulation of epidermal growth factor receptor signaling pathway
- GO:0048011 nerve growth factor receptor signaling pathway
- Regulatory
- For research purposes only
Applications of ENDOPHILIN-A1 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/500 | 1/2000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- Rabbit anti endophilin-A1 antibody detects a band of approximately 42 kDa in various cell lysates under reducing conditions
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml |
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