Rabbit anti ELK-1 (pSer389) antibody
recognizes ELK-1, also known as ETS domain-containing protein Elk-1, when phosphorylated at serine 389.
ELK-1 is a member of the ternary complex factor (TCF) family of transcription factors, and plays a role in mediating gene expression in response to growth factors.
ELK-1 is phosphorylated at serine 383 and 389 in its C-terminal region by mitogen activated protein (MAP) kinase. Phosphorylation of serine 383 and 389 are required for activation of ELK1 (Li et al. 2003
- Target Species
- Species Cross-Reactivity
|Target Species||Cross Reactivity|
||Expected from Sequence
||Expected from Sequence
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to ELK-1 (pSer389) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide
- Phospho specific-peptide corresponding to residues surrounding serine 389 of human ELK-1
- Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
MyD88-dependent toll-like receptor signaling pathway
MyD88-independent toll-like receptor signaling pathway
sequence-specific DNA binding transcription factor activity
Toll signaling pathway
transcription activator activity
toll-like receptor 1 signaling pathway
toll-like receptor 2 signaling pathway
toll-like receptor 3 signaling pathway
toll-like receptor 4 signaling pathway
sequence-specific DNA binding
innate immune response
positive regulation of transcription from RNA polymerase II promoter
nerve growth factor receptor signaling pathway
stress-activated MAPK cascade
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of ELK-1 (pSer389) antibody
|Immunohistology - Paraffin
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Useful Reagents Available
Product Specific References
Li, Q.J. et al. (2003) MAP kinase phosphorylation-dependent activation of Elk-1 leads to activation of the co-activator p300.
EMBO J. 22 (2): 281-91.