ELK-1 (pSer383) antibody
Rabbit anti ELK-1 (pSer383) antibody recognizes ELK-1, also known as ETS domain-containing protein Elk-1, when phosphorylated at serine 383.
ELK-1 is a member of the ternary complex factor (TCF) family of transcription factors, and plays a role in mediating gene expression in response to growth factors.
ELK-1 is phosphorylated at serine 383 and 389 in its C-terminal region by mitogen activated protein (MAP) kinase. Phosphorylation of serine 383 and 389 is important for activation of ELK1 (Li et al. 2003).
ELK-1 is a member of the ternary complex factor (TCF) family of transcription factors, and plays a role in mediating gene expression in response to growth factors.
ELK-1 is phosphorylated at serine 383 and 389 in its C-terminal region by mitogen activated protein (MAP) kinase. Phosphorylation of serine 383 and 389 is important for activation of ELK1 (Li et al. 2003).
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Expected from Sequence Rat Expected from Sequence - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to ELK-1 (pSer383) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide
50% Glycerol - Immunogen
- Phospho specific-peptide corresponding to residues surrounding serine 383 of human ELK-1
Storage Information
- Storage
- Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P19419
- Entrez Gene
- ELK1
- GO Terms
- GO:0005515 protein binding
- GO:0002755 MyD88-dependent toll-like receptor signaling pathway
- GO:0002756 MyD88-independent toll-like receptor signaling pathway
- GO:0003700 sequence-specific DNA binding transcription factor activity
- GO:0005634 nucleus
- GO:0008063 Toll signaling pathway
- GO:0016563 transcription activator activity
- GO:0034130 toll-like receptor 1 signaling pathway
- GO:0034134 toll-like receptor 2 signaling pathway
- GO:0034138 toll-like receptor 3 signaling pathway
- GO:0034142 toll-like receptor 4 signaling pathway
- GO:0043565 sequence-specific DNA binding
- GO:0045087 innate immune response
- GO:0045944 positive regulation of transcription from RNA polymerase II promoter
- GO:0048011 nerve growth factor receptor signaling pathway
- GO:0051403 stress-activated MAPK cascade
- Regulatory
- For research purposes only
Applications of ELK-1 (pSer383) antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunohistology - Paraffin | 1/50 | 1/100 | |
| Western Blotting | 1/500 | 1/2000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
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| Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | F IF WB | 0.1 mg | ||
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Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml |
Product Specific References
Further Reading
-
Li, Q.J. et al. (2003) MAP kinase phosphorylation-dependent activation of Elk-1 leads to activation of the co-activator p300.
EMBO J. 22 (2): 281-91.
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