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ELK-1 (pSer383) antibody

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Anti ELK-1 (pSer383) Antibody thumbnail image 1 Anti ELK-1 (pSer383) Antibody thumbnail image 2
Anti ELK-1 (pSer383) Antibody gallery image 1
Western blot analysis of extracts from HT29 cells untreated or treated with UV (for 15-30 minutes) using Rabbit anti ELK-1 (pSer383) antibody
Anti ELK-1 (pSer383) Antibody gallery image 2
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Rabbit anti ELK-1 (pSer383) antibody

Rabbit anti ELK-1 (pSer383)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP2599
Datasheet
P WB
SDS
50 µl loader

Rabbit anti ELK-1 (pSer383) antibody recognizes ELK-1, also known as ETS domain-containing protein Elk-1, when phosphorylated at serine 383.

ELK-1 is a member of the ternary complex factor (TCF) family of transcription factors, and plays a role in mediating gene expression in response to growth factors.

ELK-1 is phosphorylated at serine 383 and 389 in its C-terminal region by mitogen activated protein (MAP) kinase. Phosphorylation of serine 383 and 389 is important for activation of ELK1 (Li et al. 2003).

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse Expected from Sequence
Rat Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to ELK-1 (pSer383) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide
50% Glycerol
Immunogen
Phospho specific-peptide corresponding to residues surrounding serine 383 of human ELK-1

Storage Information

Storage
Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
P19419
Entrez Gene
ELK1
GO Terms
GO:0005515 protein binding
GO:0002755 MyD88-dependent toll-like receptor signaling pathway
GO:0002756 MyD88-independent toll-like receptor signaling pathway
GO:0003700 sequence-specific DNA binding transcription factor activity
GO:0005634 nucleus
GO:0008063 Toll signaling pathway
GO:0016563 transcription activator activity
GO:0034130 toll-like receptor 1 signaling pathway
GO:0034134 toll-like receptor 2 signaling pathway
GO:0034138 toll-like receptor 3 signaling pathway
GO:0034142 toll-like receptor 4 signaling pathway
GO:0043565 sequence-specific DNA binding
GO:0045087 innate immune response
GO:0045944 positive regulation of transcription from RNA polymerase II promoter
GO:0048011 nerve growth factor receptor signaling pathway
GO:0051403 stress-activated MAPK cascade
Regulatory
For research purposes only

Applications of ELK-1 (pSer383) antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1/50 1/100
Western Blotting 1/500 1/2000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA F IF WB 0.1 mg

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
TidyBlot Western Blot Detection Reagent:HRP STAR209P WB* 0.5 ml

Product Specific References

Further Reading

  1. Li, Q.J. et al. (2003) MAP kinase phosphorylation-dependent activation of Elk-1 leads to activation of the co-activator p300.
    EMBO J. 22 (2): 281-91.

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