Rabbit anti ELK-1 (pSer383) antibody
recognizes ELK-1, also known as ETS domain-containing protein Elk-1, when phosphorylated at serine 383.
ELK-1 is a member of the ternary complex factor (TCF) family of transcription factors, and plays a role in mediating gene expression in response to growth factors.
ELK-1 is phosphorylated at serine 383 and 389 in its C-terminal region by mitogen activated protein (MAP) kinase. Phosphorylation of serine 383 and 389 is important for activation of ELK1 (Li et al. 2003
- Target Species
- Species Cross-Reactivity
|Target Species||Cross Reactivity|
||Expected from Sequence
||Expected from Sequence
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to ELK-1 (pSer383) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide
- Phospho specific-peptide corresponding to residues surrounding serine 383 of human ELK-1
- Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
MyD88-dependent toll-like receptor signaling pathway
MyD88-independent toll-like receptor signaling pathway
sequence-specific DNA binding transcription factor activity
Toll signaling pathway
transcription activator activity
toll-like receptor 1 signaling pathway
toll-like receptor 2 signaling pathway
toll-like receptor 3 signaling pathway
toll-like receptor 4 signaling pathway
sequence-specific DNA binding
innate immune response
positive regulation of transcription from RNA polymerase II promoter
nerve growth factor receptor signaling pathway
stress-activated MAPK cascade
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of ELK-1 (pSer383) antibody
|Immunohistology - Paraffin
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Useful Reagents Available
Product Specific References
Li, Q.J. et al. (2003) MAP kinase phosphorylation-dependent activation of Elk-1 leads to activation of the co-activator p300.
EMBO J. 22 (2): 281-91.
How to Use the Spectraviewer?
Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
Select the lasers and filters you wish to include
Select combined or multi-laser view to visualize the spectra