EIF6 antibody

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Rabbit anti EIF6

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP2732
Datasheet
WB
SDS
50 µl loader

Rabbit anti EIF6 antibody recognizes eukaryotic translation initiation factor 6, also known as B(2)GCN homolog, B4 integrin interactor, CAB, p27 (BBP). In mammalian cells, it is primarily localized in the cytoplasm were it is bound to free ribosome subunit 60S. Release of EIF6 from the 60S subunit allows 80S ribosome assembly (Ceci et al. 2003). EIF6 is overexpressed in various human cancers (Zhu et al. 2017), including human colorectal cancer (Sanvito et al. 2000) and ovarian serous adenocarcinoma (Flavin et al. 2008).

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to EIF6 was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide
50% Glycerol
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-245 of human EIF6

Storage Information

Storage
Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
P56537
Entrez Gene
EIF6
GO Terms
GO:0005515 protein binding
GO:0003743 translation initiation factor activity
GO:0005737 cytoplasm
GO:0005730 nucleolus
GO:0042256 mature ribosome assembly
GO:0043022 ribosome binding
Regulatory
For research purposes only

Applications of EIF6 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/500 1/2000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
Rabbit anti EIF6 antibody detects a band of approximately 27 kDa in various cell lysates under reducing conditions

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA F IF WB 0.1 mg