Rabbit anti EIF4G1 (pSer1232) antibody
recognizes serine/threonine-protein kinase D1, also known as eIF-4-gamma 1, eIF-4G 1, p220, when phosphorylated at serine 1232. During protein synthesis, interaction between eIF4G and eIF4E is essential for the formation of the eIF4F complex (Gingras et al. 2004
). In addition, eIF4G enhances binding of eIF4E to the mRNA 5' cap (Haghighat and Sonenberg 1997
). Increased eIF4G1 expression in prostate cancer samples in associated with tumor progression (Jaiswal et al. 2018
). Phosphorylation of eIF4G1 on serine 1232 is mediated through mitogen-activated protein kinase (MAPK) (Gluck et al. 2018
- Target Species
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to EIF4G1 (pSer1232) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide
- Phospho specific-peptide corresponding to residues surrounding serine 1232 of human EIF4G1
- Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
nuclear-transcribed mRNA poly(A) tail shortening
translation initiation factor activity
regulation of translational initiation
insulin receptor signaling pathway
eukaryotic translation initiation factor 4F complex
interspecies interaction between organisms
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of EIF4G1 (pSer1232) antibody
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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Secondary Antibodies Available