EIF4G1 (pSer1232) antibody

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Rabbit anti EIF4G1 (pSer1232)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP2745
Datasheet
WB
MSDS
50 µl loader

Rabbit anti EIF4G1 (pSer1232) antibody recognizes serine/threonine-protein kinase D1, also known as eIF-4-gamma 1, eIF-4G 1, p220, when phosphorylated at serine 1232. During protein synthesis, interaction between eIF4G and eIF4E is essential for the formation of the eIF4F complex (Gingras et al. 2004). In addition, eIF4G enhances binding of eIF4E to the mRNA 5' cap (Haghighat and Sonenberg 1997). Increased eIF4G1 expression in prostate cancer samples in associated with tumor progression (Jaiswal et al. 2018). Phosphorylation of eIF4G1 on serine 1232 is mediated through mitogen-activated protein kinase (MAPK) (Gluck et al. 2018).

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to EIF4G1 (pSer1232) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide
50% Glycerol
Immunogen
Phospho specific-peptide corresponding to residues surrounding serine 1232 of human EIF4G1

Storage Information

Storage
Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
Q04637
Entrez Gene
EIF4G1
GO Terms
GO:0005515 protein binding
GO:0000289 nuclear-transcribed mRNA poly(A) tail shortening
GO:0003743 translation initiation factor activity
GO:0005829 cytosol
GO:0006446 regulation of translational initiation
GO:0008286 insulin receptor signaling pathway
GO:0016281 eukaryotic translation initiation factor 4F complex
GO:0044419 interspecies interaction between organisms
Regulatory
For research purposes only

Applications of EIF4G1 (pSer1232) antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/500 1/2000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA F IF WB 0.1 mg