EIF4E (pSer209) antibody

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Rabbit anti EIF4E (pSer209)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP2598 P WB 50 µl
Rabbit anti EIF4E (pSer209) antibody recognizes the eukaryotic translation initiation factor 4E (EIF4E), also known as eIF-4F 25 kDa subunit or mRNA cap-binding protein, when phosphorylated at serine 209. EIF4E is a member of the eukaryotic initiation factor 4E protein family and is the least abundant component of the eukaryotic translation initiation factor 4F complex (eIF-4F). EIF4E binds to the 5′-cap structure on eukaryotic mRNAs and is phosphorylated at serine 209 by (MAPK) signal-integrating kinase 1 (Mnk1) and (MAPK) signal-integrating kinase 2 (Mnk2) (Scheper et al. 2001).

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to EIF4E (pSer209) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide
50% Glycerol
Immunogen
Phospho specific-peptide corresponding to residues surrounding serine 209 of human EIF4E

Storage Information

Storage
Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch

More Information

UniProt
P06730 Related reagents
Entrez Gene
EIF4E Related reagents
GO Terms
GO:0000082 G1/S transition of mitotic cell cycle
GO:0005515 protein binding
GO:0000289 nuclear-transcribed mRNA poly(A) tail shortening
GO:0000339 RNA cap binding
GO:0003743 translation initiation factor activity
GO:0005829 cytosol
GO:0005845 mRNA cap binding complex
GO:0006406 mRNA export from nucleus
GO:0006417 regulation of translation
GO:0008286 insulin receptor signaling pathway
GO:0016281 eukaryotic translation initiation factor 4F complex
GO:0044419 interspecies interaction between organisms
GO:0045931 positive regulation of mitotic cell cycle
Regulatory
For research purposes only

Applications of EIF4E (pSer209) antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1/50 1/100
Western Blotting 1/500 1/2000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
Due to the presence of phosphoproteins in milk, the use of milk based blocking reagents is not recommended. 1% BSA in PBS or TBS Tween should be used instead.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
TidyBlot™ Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*

Product Specific References

Further Reading

  1. Scheper, G.C. et al. (2001) The mitogen-activated protein kinase signal-integrating kinase Mnk2 is a eukaryotic initiation factor 4E kinase with high levels of basal activity in mammalian cells.
    Mol Cell Biol. 21 (3): 743-54.