Rabbit anti EIF4E (pSer209) antibody
recognizes the eukaryotic translation initiation factor 4E (EIF4E), also known as eIF-4F 25 kDa subunit or mRNA cap-binding protein, when phosphorylated at serine 209. EIF4E is a member of the eukaryotic initiation factor 4E protein family and is the least abundant component of the eukaryotic translation initiation factor 4F complex (eIF-4F). EIF4E binds to the 5′-cap structure on eukaryotic mRNAs and is phosphorylated at serine 209 by (MAPK) signal-integrating kinase 1 (Mnk1) and (MAPK) signal-integrating kinase 2 (Mnk2) (Scheper et al. 2001
- Target Species
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to EIF4E (pSer209) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide
- Phospho specific-peptide corresponding to residues surrounding serine 209 of human EIF4E
- Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
G1/S transition of mitotic cell cycle
nuclear-transcribed mRNA poly(A) tail shortening
RNA cap binding
translation initiation factor activity
mRNA cap binding complex
mRNA export from nucleus
regulation of translation
insulin receptor signaling pathway
eukaryotic translation initiation factor 4F complex
interspecies interaction between organisms
positive regulation of mitotic cell cycle
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of EIF4E (pSer209) antibody
|Immunohistology - Paraffin
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Useful Reagents Available
Product Specific References
Scheper, G.C. et al. (2001) The mitogen-activated protein kinase signal-integrating kinase Mnk2 is a eukaryotic initiation factor 4E kinase with high levels of basal activity in mammalian cells.
Mol Cell Biol. 21 (3): 743-54.
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