EIF4B (pSer422) antibody

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Rabbit anti EIF4B (pSer422)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP2744
Datasheet
WB
MSDS
50 µl loader

Rabbit anti EIF4B (pSer422) antibody recognizes eukaryotic translation initiation factor 4B, also known as eIF-4B, when phosphorylated at serine 422. EIF4B is important in protein synthesis and plays role in recruiting the 40S ribosomal subunit to the mRNA (Shahbazian et al. 2006). Phosphorylation of EIF4B on serine 422 was demonstrated to be mediated by S6 kinase (Raught et al. 2004, Shahbazian et al. 2006).

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to EIF4B (pSer422) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide
50% Glycerol
Immunogen
Phospho specific-peptide corresponding to residues surrounding serine 422 of human EIF4B

Storage Information

Storage
Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
P23588
Entrez Gene
EIF4B
GO Terms
GO:0000166 nucleotide binding
GO:0000289 nuclear-transcribed mRNA poly(A) tail shortening
GO:0003743 translation initiation factor activity
GO:0005829 cytosol
GO:0006446 regulation of translational initiation
GO:0008286 insulin receptor signaling pathway
GO:0016281 eukaryotic translation initiation factor 4F complex
Regulatory
For research purposes only

Applications of EIF4B (pSer422) antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/500 1/2000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
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Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
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Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA F IF WB 0.1 mg