EIF2S2 antibody
Rabbit anti EIF2S2
Rabbit anti-human EIF2S2 antibody recognizes eukaryotic translation initiation factor 2 subunit 2, also known as protein phosphatase 1 or regulatory subunit 67. This antibody has been extensively validated for western blotting using whole cell lysates.
EIF2S2 Antibody with Control Lysate
Rabbit anti-human EIF2S2 antibody recognizes eukaryotic translation initiation factor 2 subunit 2, also known as protein phosphatase 1 or regulatory subunit 67. This antibody has been extensively validated for western blotting using whole cell lysates.
- Product Type
- PrecisionAb™ Polyclonal
- Isotype
- Polyclonal IgG
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VPA00528 | WB | 100 µl | ||||
| VPA00528KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Rabbit anti Human EIF2S2 antibody recognizes EIF2S2 also known as eukaryotic translation initiation factor 2 subunit 2, eukaryotic translation initiation factor 2 subunit beta, protein phosphatase 1 regulatory subunit 67.
Eukaryotic translation initiation factor 2 (EIF-2) functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA and binding to a 40S ribosomal subunit. EIF-2 is composed of three subunits, alpha, beta, and gamma, with the protein encoded by EIF2S2 gene representing the beta subunit. The beta subunit catalyzes the exchange of GDP for GTP, which recycles the EIF-2 complex for another round of initiation (provided by RefSeq, Jul 2008).
Rabbit anti Human EIF2S2 antibody detects a band of 50 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Eukaryotic translation initiation factor 2 (EIF-2) functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA and binding to a 40S ribosomal subunit. EIF-2 is composed of three subunits, alpha, beta, and gamma, with the protein encoded by EIF2S2 gene representing the beta subunit. The beta subunit catalyzes the exchange of GDP for GTP, which recycles the EIF-2 complex for another round of initiation (provided by RefSeq, Jul 2008).
Rabbit anti Human EIF2S2 antibody detects a band of 50 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti EIF2S2 detects a band of approximately 50 kDa in HeLa cell lysates.
- Species Cross-Reactivity
-
Target Species Cross Reactivity Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid.
- Product Form
- Purified IgG - liquid.
- Preparation
- Rabbit polyclonal antibody purified by affinity chromatography.
- Preparation
- 20μl Rabbit polyclonal antibody purified by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline.
- Buffer Solution
- Phosphate buffered saline.
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
2% Sucrose. - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
2% Sucrose. - Immunogen
- Synthetic peptide directed towards the N terminal region of human EIF2S2
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol.
Positive Control Lysate
- Lysate Composition
- 400μg HeLa lysate lyophilized in RIPA buffer.
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20°C, avoiding repeated freeze thaw cycles.
- Storage
- Antibody: Store undiluted at -20°C, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch.
- Shelf Life
- As supplied, 12 months from date of despatch.
More Information
- UniProt
- P20042 Related reagents
- Entrez Gene
- EIF2S2 Related reagents
- GO Terms
- GO:0005515 protein binding
- GO:0003743 translation initiation factor activity
- GO:0005829 cytosol
- GO:0046872 metal ion binding
- GO:0005850 eukaryotic translation initiation factor 2 complex
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only.
Applications of EIF2S2 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB | ||
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB |
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