EIF2S2 antibody

Rabbit anti EIF2S2

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Specificity
EIF2S2

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VPA00528
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Rabbit anti Human EIF2S2 antibody recognizes EIF2S2 also known as eukaryotic translation initiation factor 2 subunit 2, eukaryotic translation initiation factor 2 subunit beta, protein phosphatase 1 regulatory subunit 67.

Eukaryotic translation initiation factor 2 (EIF-2) functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA and binding to a 40S ribosomal subunit. EIF-2 is composed of three subunits, alpha, beta, and gamma, with the protein encoded by EIF2S2 gene representing the beta subunit. The beta subunit catalyzes the exchange of GDP for GTP, which recycles the EIF-2 complex for another round of initiation (provided by RefSeq, Jul 2008).

Rabbit anti Human EIF2S2 antibody detects a band of 50 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti EIF2S2 detects a band of approximately 50 kDa in HeLa cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid.
Preparation
Rabbit polyclonal antibody purified by affinity chromatography.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
2% Sucrose.
Immunogen
Synthetic peptide directed towards the N terminal region of human EIF2S2
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

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UniProt
P20042
Entrez Gene
EIF2S2
GO Terms
GO:0005515 protein binding
GO:0003743 translation initiation factor activity
GO:0005829 cytosol
GO:0046872 metal ion binding
GO:0005850 eukaryotic translation initiation factor 2 complex
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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