EIF2S1 (pSer51) antibody

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Rabbit anti EIF2S1 (pSer51)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP2746
Datasheet
WB
MSDS
50 µl loader

Rabbit anti EIF2S1 (pSer51) antibody recognizes eukaryotic translation initiation factor 2 subunit 1 (EIF2S1) when phosphorylated on serine 51. EIF2S1 functions in protein synthesis; it binds GTP and Met-tRNAi and transfers Met-tRNAi to the 40S ribosomal subunit (Kimball 1999). EIF2S1 phosphorylation at serine 51 is a determinant of survival and adaptation of glucose-deficient cells (Muaddi et al. 2010). It has been also demonstrated that EIF2S1 phosphorylated at serine 51 acts as a molecular switch that determines the cell fate decisions by activated Akt in response to activated stress (Rajesh et al. 2015).

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to EIF2S1 (pSer51) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide
50% Glycerol
Immunogen
Phospho specific-peptide corresponding to residues surrounding serine 51 of human EIF2S1

Storage Information

Storage
Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
P05198
Entrez Gene
EIF2S1
GO Terms
GO:0005515 protein binding
GO:0003743 translation initiation factor activity
GO:0005829 cytosol
GO:0005844 polysome
GO:0005850 eukaryotic translation initiation factor 2 complex
GO:0043022 ribosome binding
Regulatory
For research purposes only

Applications of EIF2S1 (pSer51) antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/500 1/2000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA F IF WB 0.1 mg