EIF2AK2 (pThr446) antibody

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Rabbit anti EIF2AK2 (pThr446)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP2748
Datasheet
WB
SDS
50 µl loader

Rabbit anti EIF2AK2 (pThr446) antibody recognizes interferon-induced, double-stranded RNA-activated protein kinase when phosphorylated on threonine 446. EIF2AK2 is a serine/threonine protein kinase which is activated by double-stranded RNA (dsRNA) and phosphorylates the α subunit of eukaryotic initiation factor 2 (eIF2α) (Hershey 1991). EIF2AK2 is induced by kisspeptin and its activation inhibits cancer growth (Kim and Cho 2017). Autophosphorylation of EIF2AK2 at threonine 446 is critical for kinase activity (Zhang et al. 2001).

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to EIF2AK2 (pThr446) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide
50% Glycerol
Immunogen
Phospho specific-peptide corresponding to residues surrounding serine threonine 446 of human EIF2AK2

Storage Information

Storage
Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
P19525
Entrez Gene
EIF2AK2
GO Terms
GO:0005515 protein binding
GO:0003725 double-stranded RNA binding
GO:0009615 response to virus
GO:0005829 cytosol
GO:0004694 eukaryotic translation initiation factor 2alpha kinase activity
GO:0005524 ATP binding
GO:0008601 protein phosphatase type 2A regulator activity
GO:0019054 modulation by virus of host cellular process
GO:0019058 viral infectious cycle
GO:0030683 evasion by virus of host immune response
GO:0033689 negative regulation of osteoblast proliferation
GO:0046777 protein autophosphorylation
Regulatory
For research purposes only

Applications of EIF2AK2 (pThr446) antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/500 1/2000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA F IF WB 0.1 mg