Rabbit anti EIF2AK2 (pThr446) antibody
recognizes interferon-induced, double-stranded RNA-activated protein kinase when phosphorylated on threonine 446. EIF2AK2 is a serine/threonine protein kinase which is activated by double-stranded RNA (dsRNA) and phosphorylates the α subunit of eukaryotic initiation factor 2 (eIF2α) (Hershey 1991
). EIF2AK2 is induced by kisspeptin and its activation inhibits cancer growth (Kim and Cho 2017
). Autophosphorylation of EIF2AK2 at threonine 446 is critical for kinase activity (Zhang et al. 2001
- Target Species
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to EIF2AK2 (pThr446) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide
- Phospho specific-peptide corresponding to residues surrounding serine threonine 446 of human EIF2AK2
- Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
double-stranded RNA binding
response to virus
eukaryotic translation initiation factor 2alpha kinase activity
protein phosphatase type 2A regulator activity
modulation by virus of host cellular process
viral infectious cycle
evasion by virus of host immune response
negative regulation of osteoblast proliferation
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of EIF2AK2 (pThr446) antibody
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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Secondary Antibodies Available