EGR1 antibody
Rabbit anti EGR1 antibody recognizes human Early Growth Response Protein 1. EGR1 is a ~58 kDa nuclear protein belonging to the EGR family of C2H2-type zinc-finger proteins.
EGR1 functions as a transcriptional regulator. The products of target genes it activates are required for differentiation and mitogenesis. Research has also demonstrated that EGR1 expression is elevated in a range of cancers including prostate cancer where EGR1 has been shown to promote the growth of tumor cells.
EGR1 functions as a transcriptional regulator. The products of target genes it activates are required for differentiation and mitogenesis. Research has also demonstrated that EGR1 expression is elevated in a range of cancers including prostate cancer where EGR1 has been shown to promote the growth of tumor cells.
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Bovine Expected from Sequence Chimpanzee Expected from Sequence Dog Expected from Sequence Rhesus Monkey Expected from Sequence Sheep Expected from Sequence - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to Human EGR1 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.01% Sodium Azide (NaN3)
- Immunogen
- A synthetic peptide sequence corresponding to amino acid 94 - 108 of human EGR1.
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P18146
- Entrez Gene
- EGR1
- GO Terms
- GO:0005515 protein binding
- GO:0003700 sequence-specific DNA binding transcription factor activity
- GO:0005634 nucleus
- GO:0005737 cytoplasm
- GO:0008270 zinc ion binding
- GO:0060337 type I interferon-mediated signaling pathway
- GO:0071504 cellular response to heparin
- GO:0071506 cellular response to mycophenolic acid
- GO:0072303 positive regulation of glomerular metanephric mesangial cell proliferation
- Regulatory
- For research purposes only
Applications of EGR1 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | |||
| Immunohistology - Paraffin 1 | 10ug/ml | ||
| Western Blotting |
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Histology Positive Control Tissue
- Lung small cell carcinoma
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Antigen Retrieval Buffer, pH8.0 | BUF025A | P | 500 ml | ||
| 100x Antigen Retrieval Buffer, pH8.0 | BUF025C | P | 50 ml | ||
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml | ||
| Histar Detection System | STAR3000A | C P | 50 Tests |
Product Specific References
References for EGR1 antibody
-
Abu El-Asrar, A.M. et al (2011) Expression of high-mobility groups box-1/receptor for advanced glycation end products/osteopontin/early growth response-1 pathway in proliferative vitreoretinal epiretinal membranes
Mol Vis. 2011 Feb 17;17:508-18.
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