EGR1 antibody
EGR1 functions as a transcriptional regulator. The products of target genes it activates are required for differentiation and mitogenesis. Research has also demonstrated that EGR1 expression is elevated in a range of cancers including prostate cancer where EGR1 has been shown to promote the growth of tumor cells.
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Bovine Expected from Sequence Chimpanzee Expected from Sequence Dog Expected from Sequence Rhesus Monkey Expected from Sequence Sheep Expected from Sequence - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to Human EGR1 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.01% Sodium Azide (NaN3)
- Immunogen
- A synthetic peptide sequence corresponding to amino acid 94 - 108 of human EGR1.
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P18146
- Entrez Gene
- EGR1
- GO Terms
- GO:0005515 protein binding
- GO:0003700 sequence-specific DNA binding transcription factor activity
- GO:0005634 nucleus
- GO:0005737 cytoplasm
- GO:0008270 zinc ion binding
- GO:0060337 type I interferon-mediated signaling pathway
- GO:0071504 cellular response to heparin
- GO:0071506 cellular response to mycophenolic acid
- GO:0072303 positive regulation of glomerular metanephric mesangial cell proliferation
- Regulatory
- For research purposes only
Applications of EGR1 antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
ELISA | |||
Immunohistology - Paraffin 1 | 10ug/ml | ||
Western Blotting |
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
- Histology Positive Control Tissue
- Lung small cell carcinoma
Useful Reagents Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Antigen Retrieval Buffer, pH8.0 | BUF025A | P | 500 ml | ||
Antigen Retrieval Buffer, pH8.0 | BUF025C | P | 50 ml | ||
TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml | ||
Histar Detection System | STAR3000A | C P | 50 Tests |
Product Specific References
References for EGR1 antibody
-
Abu El-Asrar, A.M. et al (2011) Expression of high-mobility groups box-1/receptor for advanced glycation end products/osteopontin/early growth response-1 pathway in proliferative vitreoretinal epiretinal membranes
Mol Vis. 2011 Feb 17;17:508-18.
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