EGR1 antibody

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Rabbit anti EGR1

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP1939 E P* WB 50 µg
Rabbit anti EGR1 antibody recognizes human Early Growth Response Protein 1. EGR1 is a ~58 kDa nuclear protein belonging to the EGR family of C2H2-type zinc-finger proteins.

EGR1 functions as a transcriptional regulator. The products of target genes it activates are required for differentiation and mitogenesis. Research has also demonstrated that EGR1 expression is elevated in a range of cancers including prostate cancer where EGR1 has been shown to promote the growth of tumor cells.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Bovine Expected from Sequence
Chimpanzee Expected from Sequence
Dog Expected from Sequence
Rhesus Monkey Expected from Sequence
Sheep Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to Human EGR1 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Sodium Azide (NaN3)
Immunogen
A synthetic peptide sequence corresponding to amino acid 94 - 108 of human EGR1.
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P18146 Related reagents
Entrez Gene
EGR1 Related reagents
GO Terms
GO:0005515 protein binding
GO:0003700 sequence-specific DNA binding transcription factor activity
GO:0005634 nucleus
GO:0005737 cytoplasm
GO:0008270 zinc ion binding
GO:0060337 type I interferon-mediated signaling pathway
GO:0071504 cellular response to heparin
GO:0071506 cellular response to mycophenolic acid
GO:0072303 positive regulation of glomerular metanephric mesangial cell proliferation
Regulatory
For research purposes only

Applications of EGR1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Immunohistology - Paraffin 1 10ug/ml
Western Blotting
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Lung small cell carcinoma

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A 500 ml P
Antigen Retrieval Buffer, pH8.0 BUF025C 50 ml P
TidyBlot Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*
Histar Detection System STAR3000A 50 Tests C P
Histar Detection System STAR3000B 150 Tests C P
Histar Detection System STAR3000C 500 Tests C P

Application Based External Images

Immunohistology - Paraffin

Product Specific References

References for EGR1 antibody

  1. Abu El-Asrar, A.M. et al (2011) Expression of high-mobility groups box-1/receptor for advanced glycation end products/osteopontin/early growth response-1 pathway in proliferative vitreoretinal epiretinal membranes
    Mol Vis. 2011 Feb 17;17:508-18.