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EGF R (pTyr845) antibody

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Anti EGF R (pTyr845) Antibody gallery image 1
Western blot analysis of extracts from MDA-MB-231 cells untreated or treated with EGF using Rabbit anti EGF R (pTyr845) antibody. Cells were cultured in serum free media for 16-20 hours before treatment with EGF (100ng/ml) for 30 minutes

Rabbit anti EGF R (pTyr845)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP2597
Datasheet
WB
SDS
50 µl loader

Rabbit anti EGF R (pTyr845) antibody recognizes the epidermal growth factor receptor (EGF R), also known as Proto-oncogene c-ErbB-1 or receptor tyrosine-protein kinase erbB-1, when phosphorylated at tyrosine 845. EGF R is a transmembrane glycoprotein and a member of the ErbB receptor tyrosine kinase family. EGF R is a cell surface receptor for members of the epidermal growth factor family.

EGF R plays a role in numerous cellular processes such as cell proliferation and apoptosis through the activation of downstream signaling pathways (Scaltriti and Baselga 2006).

EGF R phosphorylation at tyrosine 845 is mediated by Src (Biscardi et al. 1999, Fu et al. 2008).

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to EGF R (pTyr845) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide
50% Glycerol
Immunogen
Phospho specific-peptide corresponding to residues surrounding tyrosine 845 of human EGF R

Storage Information

Storage
Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
P00533
Entrez Gene
EGFR
Regulatory
For research purposes only

Applications of EGF R (pTyr845) antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/500 1/1000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA F IF WB 0.1 mg

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
TidyBlot Western Blot Detection Reagent:HRP STAR209P WB* 0.5 ml

Product Specific References

Further Reading

  1. Scaltriti, M. & Baselga, J. (2006) The epidermal growth factor receptor pathway: a model for targeted therapy.
    Clin Cancer Res. 12 (18): 5268-72.
  2. Biscardi, J.S. et al. (1999) c-Src-mediated phosphorylation of the epidermal growth factor receptor on Tyr845 and Tyr1101 is associated with modulation of receptor function.
    J Biol Chem. 274 (12): 8335-43.
  3. Fu, Y.N. et al. (2008) EGFR mutants found in non-small cell lung cancer show different levels of sensitivity to suppression of Src: implications in targeting therapy.
    Oncogene. 27 (7): 957-65.

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