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EGF R (pTyr1068) antibody

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Anti EGF R (pTyr1068) Antibody thumbnail image 1 Anti EGF R (pTyr1068) Antibody thumbnail image 2 Anti EGF R (pTyr1068) Antibody thumbnail image 3
Anti EGF R (pTyr1068) Antibody gallery image 1
Multiplex Western blot analysis of Rabbit anti EFGR and Mouse anti EGFR. A431 lysates (30 μg) were loaded into lanes 2 and 5 and Pervanadate treated A431 lysates (30 μg) were loaded into lanes 3 and 6 were run under reducing conditions on SDS PAGE using the Bio-Rad V3 system and transferred to a Low Fluorescence PVDF membrane. Bio-Rad Precision Plus molecular weight marker was run in lane 1. A lambda protein phosphatase treatment was administered to lanes 4 and 6 of the membrane post-transfer. As a loading control, lanes 2, 3. 5 and 6 were probed with Human anti Human Actin Beta: Dylight 680 (HCA147) (1/200). Lanes 2, 3, 5 and 6 were also probed with Goat anti Rabbit IgG: Dylight594 (STAR36D594) (1/5000) and Goat anti Mouse IgG: Starbright Blue 520 (12005867) (1/5000) to detect Rabbit anti EGFR (pTyr1068) and Mouse anti EGFR respectively. Membranes were then visualized with the ChemiDoc MP Imaging System
Anti EGF R (pTyr1068) Antibody gallery image 2
Western Blot analysis of A431 untreated and EGF treated whole cell lysates probed with (A) Mouse anti EGF R antibody (VMA00061) or (B) Rabbit anti EGF R (pTyr1068) antibody (1/1,000, AHP2590) followed by detection with HRP conjugated Goat anti Rabbit IgG (1/10,000, STAR208P). Membranes were treated with (+) and without (-) lambda protein phosphatase and visualized on the ChemiDoc MP with a (A) 17 second or a (B) 4 second exposure. EGF R (pTyr1068) has a molecular weight of 178 kDa.
Anti EGF R (pTyr1068) Antibody gallery image 3
Normalized signal intensity of EGF R (pTyr1068) from EGF treated and untreated lysates, treated with (+) or without (-) lambda protein phosphatase. Lambda protein phosphatase dephosphorylates serine, threonine and tyrosine residues. Normalized signal intensity was calculated by dividing the TPN-Normalized signal intensity for each phosphoprotein by the TPN-normalized intensity of the corresponding total protein.
Learn more about TPN Total Protein Normalization.

Rabbit anti EGF R (pTyr1068)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP2590
Datasheet
WB
SDS
50 µl loader

Rabbit anti EGF R (pTyr1068) antibody recognizes the epidermal growth factor receptor (EGF R), also known as proto-oncogene c-ErbB-1 or receptor tyrosine-protein kinase erbB-1, when phosphorylated at tyrosine 1068. EGF R is a transmembrane glycoprotein and a member of the ErbB receptor tyrosine kinase family. EGF R is a cell surface receptor for members of the epidermal growth factor family.

EGF R plays a role in numerous cellular processes such as cell proliferation and apoptosis through the activation of downstream signaling pathways (Scaltriti and Baselga 2006).

EFG R is autophosphorylated at tyrosine 1068 upon ligand binding and receptor dimerization.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to EGF R (pTyr1068) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide
50% Glycerol
Immunogen
Phospho specific-peptide corresponding to residues surrounding tyrosine 1068 of human EGF R

Storage Information

Storage
Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
P00533
Entrez Gene
EGFR
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of EGF R (pTyr1068) antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/500 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA F IF WB 0.1 mg

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
TidyBlot Western Blot Detection Reagent:HRP STAR209P WB* 0.5 ml

Product Specific References

Further Reading

  1. Scaltriti, M. & Baselga, J. (2006) The epidermal growth factor receptor pathway: a model for targeted therapy.
    Clin Cancer Res. 12 (18): 5268-72.

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