EGF R (pTyr1068) antibody

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Anti EGF R (pTyr1068) Antibody thumbnail image 2

Anti EGF R (pTyr1068) Antibody thumbnail image 3

Anti EGF R (pTyr1068) Antibody gallery image 1 — Multiplex Western blot analysis of Rabbit anti EFGR and Mouse anti EGFR. A431 lysates (30 μg) were loaded into lanes 2 and 5 and Pervanadate treated A431 lysates (30 μg) were loaded into lanes 3 and 6 were run under reducing conditions on SDS PAGE using the Bio-Rad V3 system and transferred to a Low Fluorescence PVDF membrane. Bio-Rad Precision Plus molecular weight marker was run in lane 1. A lambda protein phosphatase treatment was administered to lanes 4 and 6 of the membrane post-transfer. As a loading control, lanes 2, 3. 5 and 6 were probed with Human anti Human Actin Beta: Dylight 680 (HCA147) (1/200). Lanes 2, 3, 5 and 6 were also probed with Goat anti Rabbit IgG: Dylight594 (STAR36D594) (1/5000) and Goat anti Mouse IgG: Starbright Blue 520 (12005867) (1/5000) to detect Rabbit anti EGFR (pTyr1068) and Mouse anti EGFR respectively. Membranes were then visualized with the ChemiDoc MP Imaging System

Anti EGF R (pTyr1068) Antibody gallery image 2 — Western Blot analysis of A431 untreated and EGF treated whole cell lysates probed with (A) Mouse anti EGF R antibody (VMA00061) or (B) Rabbit anti EGF R (pTyr1068) antibody (1/1,000, **AHP2590**) followed by detection with HRP conjugated Goat anti Rabbit IgG (1/10,000, STAR208P). Membranes were treated with (+) and without (-) lambda protein phosphatase and visualized on the ChemiDoc MP with a (A) 17 second or a (B) 4 second exposure. EGF R (pTyr1068) has a molecular weight of 178 kDa.

Anti EGF R (pTyr1068) Antibody gallery image 3 — Normalized signal intensity of EGF R (pTyr1068) from EGF treated and untreated lysates, treated with (+) or without (-) lambda protein phosphatase. Lambda protein phosphatase dephosphorylates serine, threonine and tyrosine residues. Normalized signal intensity was calculated by dividing the TPN-Normalized signal intensity for each phosphoprotein by the TPN-normalized intensity of the corresponding total protein.
Learn more about TPN Total Protein Normalization.

Rabbit anti EGF R (pTyr1068)

Product Type: Polyclonal Antibody
Isotype: Polyclonal IgG

Product Code	Applications	Pack Size	Your Price	List Price	Quantity
AHP2590 Datasheet	WB SDS	50 µl	Log in		Get Quote

Rabbit anti EGF R (pTyr1068) antibody recognizes the epidermal growth factor receptor (EGF R), also known as proto-oncogene c-ErbB-1 or receptor tyrosine-protein kinase erbB-1, when phosphorylated at tyrosine 1068. EGF R is a transmembrane glycoprotein and a member of the ErbB receptor tyrosine kinase family. EGF R is a cell surface receptor for members of the epidermal growth factor family.

EGF R plays a role in numerous cellular processes such as cell proliferation and apoptosis through the activation of downstream signaling pathways (Scaltriti and Baselga 2006).

EFG R is autophosphorylated at tyrosine 1068 upon ligand binding and receptor dimerization.

Product Details

Target Species: Human
Product Form: Purified IgG - liquid
Antiserum Preparation: Antiserum to EGF R (pTyr1068) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.02% Sodium Azide
50% Glycerol
Immunogen: Phospho specific-peptide corresponding to residues surrounding tyrosine 1068 of human EGF R

Storage Information

Storage: Store at -20^oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee: 12 months from date of despatch

More Information

UniProt: P00533
Entrez Gene: EGFR
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories
Regulatory: For research purposes only

Applications of EGF R (pTyr1068) antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/500	1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description	Product Code	Applications	Pack Size	Your Price	Quantity
Goat anti Rabbit IgG (Fc):Biotin	STAR121B	E WB	1 mg	Log in	Get Quote
Goat anti Rabbit IgG (Fc):FITC	STAR121F	F	1 mg	Log in	Get Quote
Goat anti Rabbit IgG (Fc):HRP	STAR121P	E WB	1 mg	Log in	Get Quote
Goat anti Rabbit IgG (H/L):HRP	STAR124P	C E WB	1 mg	Log in	Get Quote
Sheep anti Rabbit IgG:FITC	STAR34B	C F	1 mg	Log in	Get Quote
Sheep anti Rabbit IgG:RPE	STAR35A	F	1 ml	Log in	Get Quote
Sheep anti Rabbit IgG:DyLight®488	STAR36D488GA	F IF WB	0.1 mg	Log in	Get Quote
Sheep anti Rabbit IgG:DyLight®680	STAR36D680GA	F IF WB	0.1 mg	Log in	Get Quote
Sheep anti Rabbit IgG:Dylight®800	STAR36D800GA	F IF WB	0.1 mg	Log in	Get Quote

Useful Reagents Available

Description	Product Code	Applications	Pack Size	Your Price	List Price	Quantity
TidyBlot Western Blot Detection Reagent:HRP	STAR209P	WB*	0.5 ml	Log in		Get Quote

Product Specific References

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