EGF R (pTyr1045) antibody

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Anti EGF R (pTyr1045) Antibody gallery image 1 — Western blot analysis of extracts from A431 cells untreated or treated with EGF using Rabbit anti EGF R (pTyr1045) antibody. Cells were cultured in serum free media overnight before treatment with EGF (100ng/ml) for 30 minutes. 3% BSA was used for blocking.

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Rabbit anti EGF R (pTyr1045)

Product Type: Polyclonal Antibody
Isotype: Polyclonal IgG
Specificity: EGF R (pTyr1045)

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AHP2704 Datasheet Datasheet SDS SDS	WB	50 µl		Log in	Get Quote
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Rabbit anti EGF R (pTyr1045) antibody recognizes the epidermal growth factor receptor (EGF R), also known as proto-oncogene c-ErbB-1 or receptor tyrosine-protein kinase erbB-1, when phosphorylated at tyrosine 1045. EGF R is a transmembrane glycoprotein and a member of the ErbB receptor tyrosine kinase family. EGF R plays a role in numerous cellular processes such as cell proliferation and apoptosis through the activation of downstream signaling pathways (Scaltriti, M. & Baselga, J. 2006). The E3 ubiquitin ligase, Cbl, binds to tyrosine 1045 upon its phosphorylation (Levkowitz, G. et al. 1999) and mediates its monoubiquitination, which results in receptor endocytosis followed by endosomal/lysosomal sorting (Grøvdal, L.M. et al. 2004 and Haglund, K. & Dikic, I. 2012).

Target Species: Human
Product Form: Purified IgG - liquid
Antiserum Preparation: Antiserum to EGF R (pTyr1045) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.02% Sodium Azide
50% Glycerol
Immunogen: Phospho specific-peptide corresponding to residues surrounding tyrosine 1045 of human EGF R
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch

Store at -20^oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/500	1/2000

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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TidyBlot Western Blot Detection Reagent:HRP	STAR209P	WB*	0.5 ml	Log in	Get Quote
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Description	TidyBlot Western Blot Detection Reagent:HRP

Synonyms: Erbb; HER1
UniProt: P00533
Entrez Gene: EGFR