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EGF R antibody

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Western Blot Validated
Anti EGF Receptor Antibody (PrecisionAb Polyclonal Antibody) thumbnail image 1 Anti EGF Receptor Antibody (PrecisionAb Polyclonal Antibody) thumbnail image 2 Anti EGF Receptor Antibody (PrecisionAb Polyclonal Antibody) thumbnail image 3
Anti EGF Receptor Antibody (PrecisionAb Polyclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with EGF receptor antibody followed by detection with HRP conjugated Goat anti Rabbit IgG (1/10,000, STAR208P) and visualized on the ChemiDoc MP with 2 second exposure. Arrow points to EGF receptor (molecular weight 175 kDa)
Anti EGF Receptor Antibody (PrecisionAb Polyclonal Antibody) gallery image 2
Western blot analysis of EGF receptor KO CRISPR knockout HeLa (EGF Receptor KO) and wild type HeLa (WT) whole cell lysates probed with (A) Rabbit anti EGF receptor antibody (VPA00900) followed by detection with HRP conjugated Goat anti Rabbit IgG (H/L) (1/10,000 STAR208P) and (B) hFAB rhodamine anti-tubulin primary antibody (12004166) and visualized on the ChemiDoc MP with a (A) 342 second and (B) 30 second exposure.
Anti EGF Receptor Antibody (PrecisionAb Polyclonal Antibody) gallery image 3
Normalized signal intensity of EGF receptor from CRISPR knockout and wild type lysates. Signal intensity was normalized to the tubulin signal.

Rabbit anti EGF Receptor

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
PrecisionAb Antibodies
Product Code Applications Pack Size List Price Quantity
VPA00900
Datasheet
WB
SDS
100 µl loader

Product Details

Target Species
Human
Western Blotting
Anti EGF R antibody recognizes a band of approximately 175 kDa in A431 cell lysates
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography on immunogen
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
<50% Glycerol
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml

Storage Information

Storage
Store undiluted at -20°C, avoiding repeated freeze thaw cycles
Guarantee
12 months from date of despatch

More Information

UniProt
P00533
Entrez Gene
EGFR
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of EGF R antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P WB 2 ml

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A Precise Solution to Reproducible Western Blots - PrecisionAb Antibodies
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