EGF R antibody

Rabbit anti Human EGF Receptor

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
EGF R

Product Code Applications Pack Size List Price Your Price Qty
AHP1243
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SDS Safety Datasheet SDS
E WB 50 µg loader
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Rabbit anti Human epidermal growth factor receptor antibody recognizes human epidermal growth factor receptor (EGFR), also known as Proto-oncogene c-ErbB-1. EGFR is a 1210 amino acid single pass type 1 ~170 kDa transmembrane glycoprotein which is a member of the ErbB family of receptor tyrosine kinases. EGFR is the cell surface receptor for members of the EGF family, and functions in early embryonic development and in the renewal of stem cells in tissues such as the skin, liver and gut (Nautiyal et al. 2010).

Mutations to EGFR have been found in many human carcinomas including breast, brain, lung, and colon(Garcia de Palazzo et al. 1993).

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to EGF receptor were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Immunogen
Synthetic peptide corresponding to amino acids 1189-1199 of human EGF receptor protein.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/8000 1/32000
Western Blotting 1/500 1/5000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1243 detects a band of approximately 170kDa in A431 cell lysates.

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Further Reading

  1. Sebastian, S. et al. (2006) The complexity of targeting EGFR signalling in cancer: from expression to turnover.
    Biochim Biophys Acta. 1766 (1): 120-39.
  2. Carpenter, G. & Cohen, S. (1990) Epidermal growth factor.
    J Biol Chem. 265 (14): 7709-12.
  3. Normanno, N. et al. (2006) Epidermal growth factor receptor (EGFR) signaling in cancer.
    Gene. 366 (1): 2-16.

Synonyms
Epidermal Growth Factor Receptor
RRID
AB_844606
UniProt
P00533
Entrez Gene
EGFR
View more products with EGF R specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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