EGF R antibody

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Rabbit anti Human EGF Receptor

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP1243 E WB 50 µg
Rabbit anti Human epidermal growth factor receptor antibody recognizes human epidermal growth factor receptor (EGFR), also known as Proto-oncogene c-ErbB-1. EGFR is a 1210 amino acid single pass type 1 ~170 kDa transmembrane glycoprotein which is a member of the ErbB family of receptor tyrosine kinases. EGFR is the cell surface receptor for members of the EGF family, and functions in early embryonic development and in the renewal of stem cells in tissues such as the skin, liver and gut (Nautiyal et al. 2010).

Mutations to EGFR have been found in many human carcinomas including breast, brain, lung, and colon(Garcia de Palazzo et al. 1993).

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to EGF receptor were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01%Sodium Azide (NaN3)
Immunogen
Synthetic peptide corresponding to amino acids 1189-1199 of human EGF receptor protein.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P00533 Related reagents
Entrez Gene
EGFR Related reagents
Regulatory
For research purposes only

Applications of EGF R antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/8000 1/32000
Western Blotting 1/500 1/5000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1243 detects a band of approximately 170kDa in A431 cell lysates.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
TidyBlot™ Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*

Product Specific References

Further Reading

  1. Sebastian, S. et al. (2006) The complexity of targeting EGFR signalling in cancer: from expression to turnover.
    Biochim Biophys Acta. 1766 (1): 120-39.
  2. Carpenter, G. & Cohen, S. (1990) Epidermal growth factor.
    J Biol Chem. 265 (14): 7709-12.
  3. Normanno, N. et al. (2006) Epidermal growth factor receptor (EGFR) signaling in cancer.
    Gene. 366 (1): 2-16.