Rabbit anti EEF2K (pSer366) antibody
recognizes human eukaryotic elongation factor 2 kinase, also known as eEF-2 kinase or EF-2K, when phosphorylated at serine 366. EEF2K phosphorylates elongation factor 2 (eEF-2) within a GTP-binding domain resulting in its inactivation (Ryazanov, A.G. et al. 1988
). EEF2K is phosphorylated at serine 366 by p90RSK1 and p70 S6kα kinases, which results in inhibition of its kinase activity (Wang, X. et al. 2001
- Target Species
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to EEF2K (pSer366) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide
- Phospho specific-peptide corresponding to residues surrounding serine 366 of human EEF2K
- Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
calcium ion binding
elongation factor-2 kinase activity
translation factor activity, nucleic acid binding
insulin receptor signaling pathway
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of EEF2K (pSer366) antibody
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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Secondary Antibodies Available