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EEF2K (pSer366) antibody

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Western blot analysis of HeLa cell lysate untreated or treated with CIP using Rabbit anti EEF2K (pSer366) antibody at a 1/1000 dilution. Cells were cultured in serum free media overnight before treatment with CIP (20 μl CIP for every 400 μl lysate) at 37°C for 1 hour. 3% BSA was used for blocking

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Rabbit anti EEF2K (pSer366)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
EEF2K (pSer366)

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Rabbit anti EEF2K (pSer366) antibody recognizes human eukaryotic elongation factor 2 kinase, also known as eEF-2 kinase or EF-2K, when phosphorylated at serine 366. EEF2K phosphorylates elongation factor 2 (eEF-2) within a GTP-binding domain resulting in its inactivation (Ryazanov, A.G. et al. 1988). EEF2K is phosphorylated at serine 366 by p90RSK1 and p70 S6kα kinases, which results in inhibition of its kinase activity (Wang, X. et al. 2001).

Target Species
Human
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to EEF2K (pSer366) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide
50% Glycerol
Immunogen
Phospho specific-peptide corresponding to residues surrounding serine 366 of human EEF2K
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/500 1/2000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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UniProt
O00418
Entrez Gene
EEF2K
GO Terms
GO:0005509 calcium ion binding
GO:0005829 cytosol
GO:0004686 elongation factor-2 kinase activity
GO:0005516 calmodulin binding
GO:0005524 ATP binding
GO:0006414 translational elongation
GO:0006468 protein phosphorylation
GO:0008135 translation factor activity, nucleic acid binding
GO:0008286 insulin receptor signaling pathway
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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