EEF2K (pSer366) antibody

ISO 9001 Quality Assurance Manufacturing | Print

Anti EEF2K (pSer366) Antibody gallery image 1 — Western blot analysis of HeLa cell lysate untreated or treated with CIP using Rabbit anti EEF2K (pSer366) antibody at a 1/1000 dilution. Cells were cultured in serum free media overnight before treatment with CIP (20 μl CIP for every 400 μl lysate) at 37°C for 1 hour. 3% BSA was used for blocking

Filter by Application:

Rabbit anti EEF2K (pSer366)

Product Type: Polyclonal Antibody
Isotype: Polyclonal IgG
Specificity: EEF2K (pSer366)

Quick Links:

Secondary Antibodies
Applications
Storage Information
Batch/Lot Specific Datasheets

Antibody Quality and Performance Guarantee

Unrivaled Technical Support

Product Code Applications Pack Size List Price Your Price Qty

List Price	Your Price
AHP2706 Datasheet Datasheet SDS SDS	WB	50 µl		Log in	Get Quote
	Log in

Rabbit anti EEF2K (pSer366) antibody recognizes human eukaryotic elongation factor 2 kinase, also known as eEF-2 kinase or EF-2K, when phosphorylated at serine 366. EEF2K phosphorylates elongation factor 2 (eEF-2) within a GTP-binding domain resulting in its inactivation (Ryazanov, A.G. et al. 1988). EEF2K is phosphorylated at serine 366 by p90RSK1 and p70 S6kα kinases, which results in inhibition of its kinase activity (Wang, X. et al. 2001).

Target Species: Human
Product Form: Purified IgG - liquid
Antiserum Preparation: Antiserum to EEF2K (pSer366) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.02% Sodium Azide
50% Glycerol
Immunogen: Phospho specific-peptide corresponding to residues surrounding serine 366 of human EEF2K
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch

Store at -20^oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/500	1/2000

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Description	Product Code	Applications	Pack Size	Your Price
TidyBlot Western Blot Detection Reagent:HRP	STAR209P	WB*	0.5 ml	Log in	Get Quote
List Price	Your Price
	Log in
Description	TidyBlot Western Blot Detection Reagent:HRP

UniProt: O00418
Entrez Gene: EEF2K
GO Terms: GO:0005509 calcium ion binding; GO:0005829 cytosol; GO:0004686 elongation factor-2 kinase activity; GO:0005516 calmodulin binding; GO:0005524 ATP binding; GO:0006414 translational elongation; GO:0006468 protein phosphorylation; GO:0008135 translation factor activity, nucleic acid binding; GO:0008286 insulin receptor signaling pathway; View More GO Terms