eEF1A1 antibody

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Rabbit anti eEF1A1

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Product Code Applications Pack Size List Price Quantity
VPA00467
Datasheet
WB
SDS
100 µl loader

Rabbit anti Human eEF1A1 antibody recognizes the elongation factor 1-alpha 1, also known as CTCL tumor antigen, EF1a-like protein, cervical cancer suppressor 3, elongation factor Tu, eukaryotic elongation factor 1 A-1, eukaryotic translation elongation factor 1 alpha 1-like 14, glucocorticoid receptor AF-1 specific elongation factor, leukocyte receptor cluster (LRC) member 7 or prostate tumor-inducing protein 1.

The EEF1A1 gene encodes an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas, and the other isoform (alpha 2) is expressed in brain, heart and skeletal muscle. This isoform is identified as an autoantigen in 66% of patients with Felty syndrome (Ditzel et al.et al. 2000). EEF1A1 has been found to have multiple copies on many chromosomes, some of which, if not all, represent different pseudogenes (provided by RefSeq, Jul 2008).

Rabbit anti Human eEF1A1 antibody detects a band of 49 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti eEF1A1 detects a band of approximately 49 kDa in HEK293 cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid.
Preparation
Rabbit polyclonal antibody prepared by affinity chromatography on Protein A.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
2% Sucrose.
Immunogen
Synthetic peptide directed towards the C terminal region of human eEF1A1

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles
Guarantee
12 months from date of despatch

More Information

UniProt
P68104
Entrez Gene
EEF1A1
GO Terms
GO:0005515 protein binding
GO:0003924 GTPase activity
GO:0003746 translation elongation factor activity
GO:0005829 cytosol
GO:0005525 GTP binding
GO:0005853 eukaryotic translation elongation factor 1 complex
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only.

Applications of eEF1A1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P WB 2 ml