EBP50 antibody

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Rabbit anti EBP50

EBP50 antibody recognizes EBP50 also known as Na(+)/H(+) exchange regulatory cofactor NHE-RF1, ezrin-radixin-moesin binding phosphoprotein-50 or Na+/H+ exchange regulatory co-factor.

EBP50 Antibody with Control Lysate

EBP50 antibody recognizes EBP50 also known as Na(+)/H(+) exchange regulatory cofactor NHE-RF1, ezrin-radixin-moesin binding phosphoprotein-50 or Na+/H+ exchange regulatory co-factor.

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00781 WB 100 µl
VPA00781KT WB 2 Westerns
With Lysate 400µg
Rabbit anti Human EBP50 antibody recognizes EBP50 also known as Na(+)/H(+) exchange regulatory cofactor NHE-RF1, ezrin-radixin-moesin binding phosphoprotein-50 or Na+/H+ exchange regulatory co-factor.

This gene encodes a sodium/hydrogen exchanger regulatory cofactor. The protein interacts with and regulates various proteins including the cystic fibrosis transmembrane conductance regulator and G-protein coupled receptors such as the beta2-adrenergic receptor and the parathyroid hormone 1 receptor. The protein also interacts with proteins that function as linkers between integral membrane and cytoskeletal proteins. The protein localizes to actin-rich structures including membrane ruffles, microvilli, and filopodia. Mutations in this gene result in hypophosphatemic nephrolithiasis/osteoporosis type 2, and loss of heterozygosity of this gene is implicated in breast cancer (provided by RefSeq, Sep 2009).

Rabbit anti Human EBP50 detects a band of 50 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti EBP50 antibody recognizes a band of approximately 50 kDa in MCF7 cell lysates
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography on immunogen
Preparation
20μl Rabbit polyclonal antibody purified by affinity chromatography on immunogen
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
2% Sucrose
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
2% Sucrose
Immunogen
Synthetic peptide directed towards the C terminal region of human EBP50
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg MCF7 lysate lyophilized in RIPA buffer
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch
Shelf Life
As supplied, 12 months from date of despatch

More Information

UniProt
O14745 Related reagents
Entrez Gene
SLC9A3R1 Related reagents
GO Terms
GO:0001726 ruffle
GO:0016055 Wnt receptor signaling pathway
GO:0005813 centrosome
GO:0005902 microvillus
GO:0006461 protein complex assembly
GO:0012505 endomembrane system
GO:0015629 actin cytoskeleton
GO:0016324 apical plasma membrane
GO:0030165 PDZ domain binding
GO:0030175 filopodium
GO:0031698 beta-2 adrenergic receptor binding
GO:0032415 regulation of sodium:hydrogen antiporter activity
GO:0043231 intracellular membrane-bounded organelle
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of EBP50 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB

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