EBP50 antibody
Rabbit anti Human EBP50 antibody recognizes EBP50 also known as Na(+)/H(+) exchange regulatory cofactor NHE-RF1, ezrin-radixin-moesin binding phosphoprotein-50 or Na+/H+ exchange regulatory co-factor.
This gene encodes a sodium/hydrogen exchanger regulatory cofactor. The protein interacts with and regulates various proteins including the cystic fibrosis transmembrane conductance regulator and G-protein coupled receptors such as the beta2-adrenergic receptor and the parathyroid hormone 1 receptor. The protein also interacts with proteins that function as linkers between integral membrane and cytoskeletal proteins. The protein localizes to actin-rich structures including membrane ruffles, microvilli, and filopodia. Mutations in this gene result in hypophosphatemic nephrolithiasis/osteoporosis type 2, and loss of heterozygosity of this gene is implicated in breast cancer (provided by RefSeq, Sep 2009).
Rabbit anti Human EBP50 detects a band of 50 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
This gene encodes a sodium/hydrogen exchanger regulatory cofactor. The protein interacts with and regulates various proteins including the cystic fibrosis transmembrane conductance regulator and G-protein coupled receptors such as the beta2-adrenergic receptor and the parathyroid hormone 1 receptor. The protein also interacts with proteins that function as linkers between integral membrane and cytoskeletal proteins. The protein localizes to actin-rich structures including membrane ruffles, microvilli, and filopodia. Mutations in this gene result in hypophosphatemic nephrolithiasis/osteoporosis type 2, and loss of heterozygosity of this gene is implicated in breast cancer (provided by RefSeq, Sep 2009).
Rabbit anti Human EBP50 detects a band of 50 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti EBP50 antibody recognizes a band of approximately 50 kDa in MCF7 cell lysates
- Product Form
- Purified IgG - liquid
- Preparation
- Rabbit polyclonal antibody purified by affinity chromatography on immunogen
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
2% Sucrose - Immunogen
- Synthetic peptide directed towards the C terminal region of human EBP50
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- O14745
- Entrez Gene
- SLC9A3R1
- GO Terms
- GO:0001726 ruffle
- GO:0016055 Wnt receptor signaling pathway
- GO:0005813 centrosome
- GO:0005902 microvillus
- GO:0006461 protein complex assembly
- GO:0012505 endomembrane system
- GO:0015629 actin cytoskeleton
- GO:0016324 apical plasma membrane
- GO:0030165 PDZ domain binding
- GO:0030175 filopodium
- GO:0031698 beta-2 adrenergic receptor binding
- GO:0032415 regulation of sodium:hydrogen antiporter activity
- GO:0043231 intracellular membrane-bounded organelle
- Acknowledgements
- PrecisionAb is a trademark of Bio-Rad Laboratories
- Regulatory
- For research purposes only
Applications of EBP50 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 |
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | WB | 2 ml |
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