EBP50 antibody

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Anti EBP50 Antibody (PrecisionAb Polyclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with Rabbit anti Human EBP50 antibody (**VPA00781**) followed by detection with Goat anti Rabbit HRP conjugated (1/10,000, STAR208P) and visualized on the ChemiDoc MP with 2 second exposure. Arrow points to EBP50 (molecular weight 50 kDa).

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Rabbit anti EBP50

Product Type: PrecisionAb Polyclonal
Isotype: Polyclonal IgG
Format: Purified
Specificity: EBP50

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Product Code Applications Pack Size List Price Your Price Qty

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VPA00781 Datasheet Datasheet SDS SDS	WB	100 µl		Log in	Get Quote
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Rabbit anti Human EBP50 antibody recognizes EBP50 also known as Na(+)/H(+) exchange regulatory cofactor NHE-RF1, ezrin-radixin-moesin binding phosphoprotein-50 or Na+/H+ exchange regulatory co-factor.

This gene encodes a sodium/hydrogen exchanger regulatory cofactor. The protein interacts with and regulates various proteins including the cystic fibrosis transmembrane conductance regulator and G-protein coupled receptors such as the beta2-adrenergic receptor and the parathyroid hormone 1 receptor. The protein also interacts with proteins that function as linkers between integral membrane and cytoskeletal proteins. The protein localizes to actin-rich structures including membrane ruffles, microvilli, and filopodia. Mutations in this gene result in hypophosphatemic nephrolithiasis/osteoporosis type 2, and loss of heterozygosity of this gene is implicated in breast cancer (provided by RefSeq, Sep 2009).

Rabbit anti Human EBP50 detects a band of 50 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Anti EBP50 antibody recognizes a band of approximately 50 kDa in MCF7 cell lysates.
Product Form: Purified IgG - liquid
Preparation: Rabbit polyclonal antibody purified by affinity chromatography on immunogen.
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
2% Sucrose
Immunogen: Synthetic peptide directed towards the C terminal region of human EBP50
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting			1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Rabbit IgG (H/L):HRP	STAR208P	WB	2 ml	Log in	Get Quote
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Description	Goat anti Rabbit IgG (H/L):HRP

UniProt: O14745
Entrez Gene: SLC9A3R1
GO Terms: GO:0001726 ruffle; GO:0016055 Wnt receptor signaling pathway; GO:0005813 centrosome; GO:0005902 microvillus; GO:0006461 protein complex assembly; GO:0012505 endomembrane system; GO:0015629 actin cytoskeleton; GO:0016324 apical plasma membrane; GO:0030165 PDZ domain binding; View More GO Terms; GO:0030175 filopodium; GO:0031698 beta-2 adrenergic receptor binding; GO:0032415 regulation of sodium:hydrogen antiporter activity; GO:0043231 intracellular membrane-bounded organelle