EB1 antibody

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Anti EB1 Antibody (PrecisionAb Polyclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with EB1 antibody followed by detection with Goat anti Rabbit HRP conjugated (1/10,000, STAR208P) and visualized on the ChemiDoc MP with 30 second exposure. Arrow points to EB1 (molecular weight 30 kDa)

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Rabbit anti EB1

Product Type: PrecisionAb Polyclonal
Isotype: Polyclonal IgG
Format: Purified
Specificity: EB1

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VPA00843 Datasheet Datasheet SDS SDS	WB	100 µl		Log in	Get Quote
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Rabbit anti Human EB1 antibody recognizes EB1, also known as APC-binding protein EB1, adenomatous polyposis coli-binding protein EB1, end-binding protein 1 and microtubule-associated protein RP/EB family member 1.

The protein encoded by MAPRE1 was first identified by its binding to the APC protein which is often mutated in familial and sporadic forms of colorectal cancer. This protein localizes to microtubules, especially the growing ends, in interphase cells. During mitosis, the protein is associated with the centrosomes and spindle microtubules. The protein also associates with components of the dynactin complex and the intermediate chain of cytoplasmic dynein. Because of these associations, it is thought that this protein is involved in the regulation of microtubule structures and chromosome stability. MAPRE1 is a member of the RP/EB family (provided by RefSeq, Jul 2008).

Rabbit anti Human EB1 antibody detects a band of 30 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Anti EB1 antibody recognizes a band of approximately 30 kDa in A431 cell lysates
Species Cross-Reactivity: Target Species Cross Reactivity

Mouse; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG - liquid
Preparation: Rabbit polyclonal antibody purified by affinity chromatography on immunogen
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide
<50% Glycerol
Immunogen: Recombinant protein of human EB1
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories

Target Species	Cross Reactivity
Mouse

Store undiluted at -20°C, avoiding repeated freeze thaw cycles

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting			1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Rabbit IgG (H/L):HRP	STAR208P	WB	2 ml	Log in	Get Quote
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Description	Goat anti Rabbit IgG (H/L):HRP

UniProt: Q15691
Entrez Gene: MAPRE1
GO Terms: GO:0000086 G2/M transition of mitotic cell cycle; GO:0000236 mitotic prometaphase; GO:0005829 cytosol; GO:0005813 centrosome; GO:0008022 protein C-terminus binding; GO:0008283 cell proliferation; GO:0030981 cortical microtubule cytoskeleton; GO:0031115 negative regulation of microtubule polymerization; GO:0035372 protein localization to microtubule; View More GO Terms; GO:0051010 microtubule plus-end binding; GO:0051301 cell division