EB1 antibody
Rabbit anti EB1
EB1 antibody recognizes EB1, also known as APC-binding protein EB1, adenomatous polyposis coli-binding protein EB1 or end-binding protein 1.
EB1 Antibody with Control Lysate
EB1 antibody recognizes EB1, also known as APC-binding protein EB1, adenomatous polyposis coli-binding protein EB1 or end-binding protein 1.
- Product Type
- PrecisionAb™ Polyclonal
- Isotype
- Polyclonal IgG
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VPA00843 | WB | 100 µl | ||||
| VPA00843KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Rabbit anti Human EB1 antibody recognizes EB1, also known as APC-binding protein EB1, adenomatous polyposis coli-binding protein EB1, end-binding protein 1 and microtubule-associated protein RP/EB family member 1.
The protein encoded by MAPRE1 was first identified by its binding to the APC protein which is often mutated in familial and sporadic forms of colorectal cancer. This protein localizes to microtubules, especially the growing ends, in interphase cells. During mitosis, the protein is associated with the centrosomes and spindle microtubules. The protein also associates with components of the dynactin complex and the intermediate chain of cytoplasmic dynein. Because of these associations, it is thought that this protein is involved in the regulation of microtubule structures and chromosome stability. MAPRE1 is a member of the RP/EB family (provided by RefSeq, Jul 2008).
Rabbit anti Human EB1 antibody detects a band of 30 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
The protein encoded by MAPRE1 was first identified by its binding to the APC protein which is often mutated in familial and sporadic forms of colorectal cancer. This protein localizes to microtubules, especially the growing ends, in interphase cells. During mitosis, the protein is associated with the centrosomes and spindle microtubules. The protein also associates with components of the dynactin complex and the intermediate chain of cytoplasmic dynein. Because of these associations, it is thought that this protein is involved in the regulation of microtubule structures and chromosome stability. MAPRE1 is a member of the RP/EB family (provided by RefSeq, Jul 2008).
Rabbit anti Human EB1 antibody detects a band of 30 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti EB1 antibody recognizes a band of approximately 30 kDa in A431 cell lysates
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Rabbit polyclonal antibody purified by affinity chromatography on immunogen
- Preparation
- 20μl Rabbit polyclonal antibody purified by affinity chromatography on immunogen
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide
<50% Glycerol - Preservative Stabilisers
- 0.09% Sodium Azide
<50% Glycerol - Immunogen
- Recombinant protein of human EB1
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg A431 lysate lyophilized in RIPA buffer
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20°C, avoiding repeated freeze thaw cycles
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch
- Shelf Life
- As supplied, 12 months from date of despatch.
More Information
- UniProt
- Q15691 Related reagents
- Entrez Gene
- MAPRE1 Related reagents
- GO Terms
- GO:0000086 G2/M transition of mitotic cell cycle
- GO:0000236 mitotic prometaphase
- GO:0005829 cytosol
- GO:0005813 centrosome
- GO:0008022 protein C-terminus binding
- GO:0008283 cell proliferation
- GO:0030981 cortical microtubule cytoskeleton
- GO:0031115 negative regulation of microtubule polymerization
- GO:0035372 protein localization to microtubule
- GO:0051010 microtubule plus-end binding
- GO:0051301 cell division
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of EB1 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
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Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB | ||
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB |
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