EB1 antibody

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Rabbit anti EB1

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Product Code Applications Pack Size List Price Quantity
VPA00843
Datasheet
WB
SDS
100 µl loader

Rabbit anti Human EB1 antibody recognizes EB1, also known as APC-binding protein EB1, adenomatous polyposis coli-binding protein EB1, end-binding protein 1 and microtubule-associated protein RP/EB family member 1.

The protein encoded by MAPRE1 was first identified by its binding to the APC protein which is often mutated in familial and sporadic forms of colorectal cancer. This protein localizes to microtubules, especially the growing ends, in interphase cells. During mitosis, the protein is associated with the centrosomes and spindle microtubules. The protein also associates with components of the dynactin complex and the intermediate chain of cytoplasmic dynein. Because of these associations, it is thought that this protein is involved in the regulation of microtubule structures and chromosome stability. MAPRE1 is a member of the RP/EB family (provided by RefSeq, Jul 2008).

Rabbit anti Human EB1 antibody detects a band of 30 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti EB1 antibody recognizes a band of approximately 30 kDa in A431 cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography on immunogen
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
<50% Glycerol
Immunogen
Recombinant protein of human EB1

Storage Information

Storage
Store undiluted at -20°C, avoiding repeated freeze thaw cycles
Guarantee
12 months from date of despatch

More Information

UniProt
Q15691
Entrez Gene
MAPRE1
GO Terms
GO:0000086 G2/M transition of mitotic cell cycle
GO:0000236 mitotic prometaphase
GO:0005829 cytosol
GO:0005813 centrosome
GO:0008022 protein C-terminus binding
GO:0008283 cell proliferation
GO:0030981 cortical microtubule cytoskeleton
GO:0031115 negative regulation of microtubule polymerization
GO:0035372 protein localization to microtubule
GO:0051010 microtubule plus-end binding
GO:0051301 cell division
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of EB1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P WB 2 ml