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E3 UBIQUITIN-PROTEIN Ligase CBL (pTyr700) antibody

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Anti E3 UBIQUITIN-PROTEIN Ligase CBL (pTyr700) Antibody thumbnail image 1 Anti E3 UBIQUITIN-PROTEIN Ligase CBL (pTyr700) Antibody thumbnail image 2
Anti E3 UBIQUITIN-PROTEIN Ligase CBL (pTyr700) Antibody gallery image 1
Western blot analysis of extracts from HT-29 cells treated or untreated with UV ( for 15-30 minutes) using Rabbit anti E3 ubiqutin-protein ligase CBL (pTyr700) antibody
Anti E3 UBIQUITIN-PROTEIN Ligase CBL (pTyr700) Antibody gallery image 2
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Rabbit anti E3 ubiqutin-protein ligase CBL (pTyr700) antibody

Rabbit anti E3 UBIQUITIN-PROTEIN Ligase CBL (pTyr700)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP2585
Datasheet
P WB
SDS
50 µl loader

Rabbit anti E3 ubiquitin-protein ligase CBL (pTyr700) antibody recognizes the Casitas B-lineage lymphoma (CBL) E3 ubiquitin ligase, also known as c-CBL proto-oncogene and ring finger protein 55, when phosphorylated at tyrosine 700. The CBL protein is a RING finger E3-ubiquitin ligase that plays a key role in signal transduction through mediating monoubiquitination and endocytosis of receptor tyrosine kinases (Haglund et al. 2002, Dikic and Giordano 2003).

E3 ubiqutin-protein ligase CBL is phosphorylated at tyrosine 700 by Fyn, Yes and Syk (Feshchenko et al. 1998).

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to E3 ubiqutin-protein ligase CBL (pTyr700) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide
50% Glycerol
Immunogen
Phospho specific-peptide corresponding to residues surrounding tyrosine 700 of human E3 ubiqutin-protein ligase CBL

Storage Information

Storage
Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
P22681
Entrez Gene
CBL
GO Terms
GO:0005509 calcium ion binding
GO:0003700 sequence-specific DNA binding transcription factor activity
GO:0005829 cytosol
GO:0004842 ubiquitin-protein ligase activity
GO:0004871 signal transducer activity
GO:0005634 nucleus
GO:0007173 epidermal growth factor receptor signaling pathway
GO:0008270 zinc ion binding
GO:0017124 SH3 domain binding
GO:0042059 negative regulation of epidermal growth factor receptor signaling pathway
GO:0048260 positive regulation of receptor-mediated endocytosis
Regulatory
For research purposes only

Applications of E3 UBIQUITIN-PROTEIN Ligase CBL (pTyr700) antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1/50 1/100
Western Blotting 1/500 1/1000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA F IF WB 0.1 mg

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
TidyBlot Western Blot Detection Reagent:HRP STAR209P WB* 0.5 ml

Product Specific References

Further Reading

  1. Haglund, K. et al. (2002) Cbl-directed monoubiquitination of CIN85 is involved in regulation of ligand-induced degradation of EGF receptors.
    Proc Natl Acad Sci U S A. 99 (19): 12191-6.
  2. Dikic, I. & Giordano, S. (2003) Negative receptor signalling.
    Curr Opin Cell Biol. 15 (2): 128-35.
  3. Feshchenko, E.A. et al. (1998) Fyn, Yes, and Syk phosphorylation sites in c-Cbl map to the same tyrosine residues that become phosphorylated in activated T cells.
    J Biol Chem. 273 (14): 8323-31.
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