E3 UBIQUITIN-PROTEIN Ligase CBL (pTyr700) antibody
Rabbit anti E3 ubiquitin-protein ligase CBL (pTyr700) antibody recognizes the Casitas B-lineage lymphoma (CBL) E3 ubiquitin ligase, also known as c-CBL proto-oncogene and ring finger protein 55, when phosphorylated at tyrosine 700. The CBL protein is a RING finger E3-ubiquitin ligase that plays a key role in signal transduction through mediating monoubiquitination and endocytosis of receptor tyrosine kinases (Haglund et al. 2002, Dikic and Giordano 2003).
E3 ubiqutin-protein ligase CBL is phosphorylated at tyrosine 700 by Fyn, Yes and Syk (Feshchenko et al. 1998).
E3 ubiqutin-protein ligase CBL is phosphorylated at tyrosine 700 by Fyn, Yes and Syk (Feshchenko et al. 1998).
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to E3 ubiqutin-protein ligase CBL (pTyr700) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide
50% Glycerol - Immunogen
- Phospho specific-peptide corresponding to residues surrounding tyrosine 700 of human E3 ubiqutin-protein ligase CBL
Storage Information
- Storage
- Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 12 months from date of despatch
More Information
- UniProt
- P22681 Related reagents
- Entrez Gene
- CBL Related reagents
- GO Terms
- GO:0005509 calcium ion binding
- GO:0003700 sequence-specific DNA binding transcription factor activity
- GO:0005829 cytosol
- GO:0004842 ubiquitin-protein ligase activity
- GO:0004871 signal transducer activity
- GO:0005634 nucleus
- GO:0007173 epidermal growth factor receptor signaling pathway
- GO:0008270 zinc ion binding
- GO:0017124 SH3 domain binding
- GO:0042059 negative regulation of epidermal growth factor receptor signaling pathway
- GO:0048260 positive regulation of receptor-mediated endocytosis
- Regulatory
- For research purposes only
Applications of E3 UBIQUITIN-PROTEIN Ligase CBL (pTyr700) antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunohistology - Paraffin | 1/50 | 1/100 | |
| Western Blotting | 1/500 | 1/1000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- Due to the presence of phosphoproteins in milk, the use of milk based blocking reagents is not recommended. 1% BSA in PBS or TBS Tween should be used instead.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Sheep anti Rabbit IgG:Biotin | 2AB02B | 1 ml | C E P WB | ||
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | 1 mg | E WB | ||
| Goat anti Rabbit IgG (Fc):FITC | STAR121F | 1 mg | F | ||
| Goat anti Rabbit IgG (Fc):HRP | STAR121P | 1 mg | E WB | ||
| Goat anti Rabbit IgG (H/L):HRP | STAR124P | 1 mg | C E WB | ||
| Sheep anti Rabbit IgG:FITC | STAR34B | 1 mg | C F | ||
| Sheep anti Rabbit IgG:RPE | STAR35A | 1 ml | F | ||
| Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | 0.1 mg | F IF WB | ||
| Sheep anti Rabbit IgG:DyLight®549 | STAR36D549GA | 0.1 mg | F IF | ||
| Sheep anti Rabbit IgG:DyLight®649 | STAR36D649GA | 0.1 mg | F IF | ||
| Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | 0.1 mg | F IF WB | ||
| Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | 0.1 mg | F IF WB |
Useful Reagents Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | 0.5 ml | WB* |
Product Specific References
Further Reading
-
Haglund, K. et al. (2002) Cbl-directed monoubiquitination of CIN85 is involved in regulation of ligand-induced degradation of EGF receptors.
Proc Natl Acad Sci U S A. 99 (19): 12191-6. -
Dikic, I. & Giordano, S. (2003) Negative receptor signalling.
Curr Opin Cell Biol. 15 (2): 128-35. -
Feshchenko, E.A. et al. (1998) Fyn, Yes, and Syk phosphorylation sites in c-Cbl map to the same tyrosine residues that become phosphorylated in activated T cells.
J Biol Chem. 273 (14): 8323-31.
