Dynorphin A antibody
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Rabbit anti Human dynorphin A antibody recognizes the opioid peptide dynorphin A (Fischli et al. 1982), a cleavage product of big dynorphin, itself derived from the precursor pro-encephalin-B, also known as preprodynorphin. Rabbit anti human dynorphin A antibody binds to both dynorphin A 1-17 and 1-8 but not with other big dynorphin cleavage products such as dynorphin B, also known as rimorphin.
Dynorphin A immunoreactive cells are found in the paraventricular and supraoptic nucleus and in hippocampal mossy fibers of rat brain following kainic acid administration and colchicine induced neurotoxicity (McGinty et al. 1983). Dynorphins play a role in multiple physiologic processes including pain perception (Wang et al. 2001)and responses to stress (Rácz et al. 2013). They mimic and compete with a number of opiate drugs(Chavkin 2013). Dysregulation of dynorphin A expression has been linked to progression of neurodegenerative conditions such as Altzheimer’s disease (Yakovleva et al. 2007) and appears to play a significant role in the pathobiology of epilepsy (Loacker et al. 2007).
Rabbit anti human dynorphin antibody has been used successfully for the immunohistochemical demonstration of ‘mossy fibre’ sprouting in hippocampal sclerosis (Thom et al. 2009) and in a virally induced disease model in the rat (Solbrig et al. 2006).
Dynorphin A immunoreactive cells are found in the paraventricular and supraoptic nucleus and in hippocampal mossy fibers of rat brain following kainic acid administration and colchicine induced neurotoxicity (McGinty et al. 1983). Dynorphins play a role in multiple physiologic processes including pain perception (Wang et al. 2001)and responses to stress (Rácz et al. 2013). They mimic and compete with a number of opiate drugs(Chavkin 2013). Dysregulation of dynorphin A expression has been linked to progression of neurodegenerative conditions such as Altzheimer’s disease (Yakovleva et al. 2007) and appears to play a significant role in the pathobiology of epilepsy (Loacker et al. 2007).
Rabbit anti human dynorphin antibody has been used successfully for the immunohistochemical demonstration of ‘mossy fibre’ sprouting in hippocampal sclerosis (Thom et al. 2009) and in a virally induced disease model in the rat (Solbrig et al. 2006).
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Pig Rat Rhesus Monkey - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Serum - liquid
- Antiserum Preparation
- Antisera to Dynorphin A 1-17 were raised by repeated immunisation of rabbits with highly purified antigen. Whole serum is supplied - diluted.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 0.1% Bovine Serum Albumin - Immunogen
- Dynorphin A 1-17: Tyr-Gly-Gly-Phe-Leu-Arg-Arg-Ile-Arg-Pro-Lys-Leu-Lys-Trp-Asp-Asn-Gln (porcine)
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P01213
- Entrez Gene
- PDYN
- GO Terms
- GO:0005886 plasma membrane
- GO:0001515 opioid peptide activity
- GO:0005576 extracellular region
- GO:0007218 neuropeptide signaling pathway
- GO:0007268 synaptic transmission
- Regulatory
- For research purposes only
Applications of Dynorphin A antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunohistology - Frozen | 1/100 | 1/1500 | |
| Immunohistology - Paraffin 1 | |||
| Radioimmunoassays | 1/10,000 |
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Overnight incubation is recommended for paraffin sections.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
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| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
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| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
|
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| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
|
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| Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
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| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
|
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| Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | F IF WB | 0.1 mg |
Product Specific References
References for Dynorphin A antibody
-
Khachaturian, H. et al. (1985) Prodynorphin peptide immunocytochemistry in rhesus monkey brain.
Peptides. 6 Suppl 2: 155-66. -
Sherman, T.G. et al. (1988) Regulation of hypothalamic magnocellular neuropeptides and their mRNAs in the Brattleboro rat: coordinate responses to further osmotic challenge.
J Neurosci. 8 (10): 3785-96. -
Zardetto-smith, A.M. et al. (1988) Lateral hypothalamic dynorphinergic efferents to the amygdala and brainstem in the rat.
Peptides. 9 (5): 1121-7. -
Beck, H. et al. (2000) Synaptic plasticity in the human dentate gyrus.
J Neurosci. 20 (18): 7080-6. -
Thom, M. et al. (2009) Bilateral reorganization of the dentate gyrus in hippocampal sclerosis: a postmortem study.
Neurology. 73: 1033-40. -
Solbrig, M.V. et al. (2006) Kappa opioid control of seizures produced by a virus in an animal model.
Brain. 129: 642-54. -
Jeub, M. et al. (1999) Loss of dynorphin-mediated inhibition of voltage-dependent Ca2+ currents in hippocampal granule cells isolated from epilepsy patients is associated with mossy fiber sprouting.
Neuroscience. 94: 465-71. -
Beck, H. et al. (2000) Synaptic plasticity in the human dentate gyrus.
J Neurosci. 20: 7080-6. -
Knapp, P.E. et al. (2001) Endogenous opioids and oligodendroglial function: possible autocrine/paracrine effects on cell survival and development.
Glia. 35: 156-65. -
Dougherty, K.J. et al. (2009) Phenotypic Diversity and Expression of GABAergic Inhibitory Interneurons During Postnatal Development in Lumbar Spinal Cord of GAD67-GFP Mice.
Neuroscience. 163: 909-19.
