Dynactin 2 antibody
Rabbit anti Dynactin 2
Rabbit anti human DCTN2 antibody recognizes dynactin subunit 2, also known as dynamitin, Dynactin 2, 50 kDa dynein-associated polypeptide, 50 kDa dynein-associated polypeptide, or p50 dynamitin. This antibody has been extensively validated for western blotting using whole cell lysates.
Dynactin 2 Antibody with Control Lysate
Rabbit anti human DCTN2 antibody recognizes dynactin subunit 2, also known as dynamitin, Dynactin 2, 50 kDa dynein-associated polypeptide, 50 kDa dynein-associated polypeptide, dynactin complex 50 kDa subunit, dynactin complex 50 kDa subunit, dynactin subunit 2, or p50 dynamitin.
- Product Type
- PrecisionAb™ Polyclonal
- Isotype
- Polyclonal IgG
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VPA00263 | WB | 100 µl | ||||
| VPA00263KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Rabbit anti Human dynactin 2 antibody recognizes dynactin 2, also known as dynamitin, epididymis secretory protein Li 77 and p50 dynamitin.
The dynactin 2 gene encodes a 50 kDa subunit of dynactin, a macromolecular complex consisting of 10-11 subunits ranging in size from 22 to 150 kDa. Dynactin binds to both microtubules and cytoplasmic dynein. It is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit is present in 4-5 copies per dynactin molecule. It contains three short alpha-helical coiled-coil domains that may mediate association with self or other dynactin subunits. It may interact directly with the largest subunit (p150) of dynactin and may affix p150 in place. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for dynactin 2 (provided by RefSeq, May 2012).
Rabbit anti Human dynactin 2 antibody detects a band of 44 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
The dynactin 2 gene encodes a 50 kDa subunit of dynactin, a macromolecular complex consisting of 10-11 subunits ranging in size from 22 to 150 kDa. Dynactin binds to both microtubules and cytoplasmic dynein. It is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit is present in 4-5 copies per dynactin molecule. It contains three short alpha-helical coiled-coil domains that may mediate association with self or other dynactin subunits. It may interact directly with the largest subunit (p150) of dynactin and may affix p150 in place. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for dynactin 2 (provided by RefSeq, May 2012).
Rabbit anti Human dynactin 2 antibody detects a band of 44 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti dynactin 2 detects a band of approximately 44 kDa in MCF7 cell lysate
- Product Form
- Ig fraction - liquid
- Product Form
- Ig fraction - liquid
- Preparation
- Rabbit Ig fraction prepared by ammonium sulphate precipitation
- Preparation
- 20μl Rabbit Ig fraction prepared by ammonium sulphate precipitation
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- KLH-conjugated synthetic peptide corresponding to aa 192-219 of human dynactin 2
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg MCF7 lysate lyophilized in RIPA buffer
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch
- Shelf Life
- As supplied, 12 months from date of despatch
More Information
- UniProt
- Q13561 Related reagents
- Entrez Gene
- DCTN2 Related reagents
- GO Terms
- GO:0000086 G2/M transition of mitotic cell cycle
- GO:0005515 protein binding
- GO:0000776 kinetochore
- GO:0003774 motor activity
- GO:0016020 membrane
- GO:0005829 cytosol
- GO:0005813 centrosome
- GO:0005869 dynactin complex
- GO:0005874 microtubule
- GO:0007067 mitosis
- GO:0008283 cell proliferation
- GO:0030286 dynein complex
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Regulatory
- For research purposes only
Applications of Dynactin 2 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
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Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB | ||
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB |
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