Dynactin 2 antibody
Rabbit anti Human dynactin 2 antibody recognizes dynactin 2, also known as dynamitin, epididymis secretory protein Li 77 and p50 dynamitin.
The dynactin 2 gene encodes a 50 kDa subunit of dynactin, a macromolecular complex consisting of 10-11 subunits ranging in size from 22 to 150 kDa. Dynactin binds to both microtubules and cytoplasmic dynein. It is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit is present in 4-5 copies per dynactin molecule. It contains three short alpha-helical coiled-coil domains that may mediate association with self or other dynactin subunits. It may interact directly with the largest subunit (p150) of dynactin and may affix p150 in place. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for dynactin 2 (provided by RefSeq, May 2012).
Rabbit anti Human dynactin 2 antibody detects a band of 44 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
The dynactin 2 gene encodes a 50 kDa subunit of dynactin, a macromolecular complex consisting of 10-11 subunits ranging in size from 22 to 150 kDa. Dynactin binds to both microtubules and cytoplasmic dynein. It is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit is present in 4-5 copies per dynactin molecule. It contains three short alpha-helical coiled-coil domains that may mediate association with self or other dynactin subunits. It may interact directly with the largest subunit (p150) of dynactin and may affix p150 in place. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for dynactin 2 (provided by RefSeq, May 2012).
Rabbit anti Human dynactin 2 antibody detects a band of 44 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti dynactin 2 detects a band of approximately 44 kDa in MCF7 cell lysate
- Product Form
- Ig fraction - liquid
- Preparation
- Rabbit Ig fraction prepared by ammonium sulphate precipitation
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- KLH-conjugated synthetic peptide corresponding to aa 192-219 of human dynactin 2
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- Q13561
- Entrez Gene
- DCTN2
- GO Terms
- GO:0000086 G2/M transition of mitotic cell cycle
- GO:0005515 protein binding
- GO:0000776 kinetochore
- GO:0003774 motor activity
- GO:0016020 membrane
- GO:0005829 cytosol
- GO:0005813 centrosome
- GO:0005869 dynactin complex
- GO:0005874 microtubule
- GO:0007067 mitosis
- GO:0008283 cell proliferation
- GO:0030286 dynein complex
- Acknowledgements
- PrecisionAb is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of Dynactin 2 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 |
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
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|---|---|---|---|---|---|
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