DRAM antibody
Rabbit anti DRAM (C-terminal) antibody recognizes human DRAM, a lysosomal modulator of autophagy that plays a central role in p53/TP53-mediated apoptosis.
DRAM is significantly down-regulated in tumor cell lines by methylation-dependent transcriptional silencing.
DRAM is significantly down-regulated in tumor cell lines by methylation-dependent transcriptional silencing.
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to human DRAM were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide (NaN3)
- Immunogen
- A peptide corresponding to a 16 amino acid sequence from near the carboxy terminus of Human DRAM.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- Q8N682
- Entrez Gene
- DRAM1
- GO Terms
- GO:0006915 apoptosis
- GO:0016021 integral to membrane
- GO:0005765 lysosomal membrane
- GO:0006914 autophagy
- Regulatory
- For research purposes only
Applications of DRAM antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunohistology - Paraffin 1 | 2.5ug/ml | ||
| Western Blotting | 0.5 | 1.0ug/ml |
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- AHP1694 detects bands of approximately 16 and 31kDa in 293 cell lysate
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Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Antigen Retrieval Buffer, pH8.0 | BUF025A | P | 500 ml | ||
| 100x Antigen Retrieval Buffer, pH8.0 | BUF025C | P | 50 ml | ||
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml |
Product Specific References
Further Reading
-
Crighton D, et al. (2007) DRAM links autophagy to p53 and programmed cell death.
Autophagy 3:72-4 -
Crighton D, et al (2006) DRAM, a p53-induced modulator of autophagy, is critical for apoptosis.
Cell 126:121-34. -
Gozuacik D., et al (2004) Autophagy as a cell death and tumor suppressor mechanism. .
Oncogene 23:2891-906
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