DRAM antibody

Rabbit anti DRAM (C-Terminal)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
DRAM
Region
(C-Terminal)

Product Code Applications Pack Size List Price Your Price Qty
AHP1694
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P * WB 0.1 mg loader
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Rabbit anti DRAM (C-terminal) antibody recognizes human DRAM, a lysosomal modulator of autophagy that plays a central role in p53/TP53-mediated apoptosis.

DRAM is significantly down-regulated in tumor cell lines by methylation-dependent transcriptional silencing.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to human DRAM were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
Immunogen
A peptide corresponding to a 16 amino acid sequence from near the carboxy terminus of Human DRAM.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1 2.5ug/ml
Western Blotting 0.5 1.0ug/ml
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1694 detects bands of approximately 16 and 31kDa in 293 cell lysate

Description Product Code Applications Pack Size List Price Your Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A P 500 ml
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Description Antigen Retrieval Buffer, pH8.0
TidyBlot Western Blot Detection Reagent:HRP STAR209P WB* 0.5 ml
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Description TidyBlot Western Blot Detection Reagent:HRP

Further Reading

  1. Crighton D, et al. (2007) DRAM links autophagy to p53 and programmed cell death.
    Autophagy 3:72-4
  2. Crighton D, et al (2006) DRAM, a p53-induced modulator of autophagy, is critical for apoptosis.
    Cell 126:121-34.
  3. Gozuacik D., et al (2004) Autophagy as a cell death and tumor suppressor mechanism. .
    Oncogene 23:2891-906

RRID
AB_10672543
UniProt
Q8N682
Entrez Gene
DRAM1
GO Terms
GO:0006915 apoptosis
GO:0016021 integral to membrane
GO:0005765 lysosomal membrane
GO:0006914 autophagy
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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