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DRAM antibody

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Anti DRAM (C-Terminal) Antibody thumbnail image 1 Anti DRAM (C-Terminal) Antibody thumbnail image 2
Anti DRAM (C-Terminal) Antibody gallery image 1
Western blot analysis of whole cell lysate from HEK293 human epithelial kidney cells probed with Rabbit anti Human DRAM (AHP1694) at 0.5(A) and 1(B) ug/ml
Anti DRAM (C-Terminal) Antibody gallery image 2
Immunohistochemical staining of human liver with Rabbit anti Human DRAM (AHP1694)

Rabbit anti DRAM (C-Terminal)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP1694 P* WB 0.1 mg
Rabbit anti DRAM (C-terminal) antibody recognizes human DRAM, a lysosomal modulator of autophagy that plays a central role in p53/TP53-mediated apoptosis.

DRAM is significantly down-regulated in tumor cell lines by methylation-dependent transcriptional silencing.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to human DRAM were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
Immunogen
A peptide corresponding to a 16 amino acid sequence from near the carboxy terminus of Human DRAM.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
Q8N682 Related reagents
Entrez Gene
DRAM1 Related reagents
GO Terms
GO:0006915 apoptosis
GO:0016021 integral to membrane
GO:0005765 lysosomal membrane
GO:0006914 autophagy
Regulatory
For research purposes only

Applications of DRAM antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1 2.5ug/ml
Western Blotting 0.5 1.0ug/ml
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1694 detects bands of approximately 16 and 31kDa in 293 cell lysate
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A 500 ml P
Antigen Retrieval Buffer, pH8.0 BUF025C 50 ml P
TidyBlot™ Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*

Product Specific References

Further Reading

  1. Crighton D, et al. (2007) DRAM links autophagy to p53 and programmed cell death.
    Autophagy 3:72-4
  2. Crighton D, et al (2006) DRAM, a p53-induced modulator of autophagy, is critical for apoptosis.
    Cell 126:121-34.
  3. Gozuacik D., et al (2004) Autophagy as a cell death and tumor suppressor mechanism. .
    Oncogene 23:2891-906
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