DRAM antibody


DRAM is significantly down-regulated in tumor cell lines by methylation-dependent transcriptional silencing.
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to human DRAM were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide (NaN3)
- Immunogen
- A peptide corresponding to a 16 amino acid sequence from near the carboxy terminus of Human DRAM.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- Q8N682
- Entrez Gene
- DRAM1
- GO Terms
- GO:0006915 apoptosis
- GO:0016021 integral to membrane
- GO:0005765 lysosomal membrane
- GO:0006914 autophagy
- Regulatory
- For research purposes only
Applications of DRAM antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Immunohistology - Paraffin 1 | 2.5ug/ml | ||
Western Blotting | 0.5 | 1.0ug/ml |
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
- Western Blotting
- AHP1694 detects bands of approximately 16 and 31kDa in 293 cell lysate
Useful Reagents Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Antigen Retrieval Buffer, pH8.0 | BUF025A | P | 500 ml | ||
Antigen Retrieval Buffer, pH8.0 | BUF025C | P | 50 ml | ||
TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml |
Product Specific References
Further Reading
-
Crighton D, et al. (2007) DRAM links autophagy to p53 and programmed cell death.
Autophagy 3:72-4 -
Crighton D, et al (2006) DRAM, a p53-induced modulator of autophagy, is critical for apoptosis.
Cell 126:121-34. -
Gozuacik D., et al (2004) Autophagy as a cell death and tumor suppressor mechanism. .
Oncogene 23:2891-906
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