DNase II antibody
Rabbit anti Human DNAase II antibody recognizes human DNase II, also known as Deoxyribonuclease II. DNAaseII is an enzyme involved in both the condensation of chromatin and the fragmentation of DNA.
DNase II is ubiquitously expressed in human tissues.
DNase II is ubiquitously expressed in human tissues.
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to human DNase II (CT) was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide (NaN3)
- Immunogen
- A peptide corresponding to amino acids near the carboxy terminus of human DNase II precursor.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- Q8WZ79
- O00115
- Entrez Gene
- DNASE2
- DNASE2B
- GO Terms
- GO:0005515 protein binding
- GO:0003677 DNA binding
- GO:0006915 apoptosis
- GO:0005764 lysosome
- GO:0004531 deoxyribonuclease II activity
- GO:0006259 DNA metabolic process
- Regulatory
- For research purposes only
Applications of DNase II antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunohistology - Paraffin 1 | 5ug/ml | ||
| Western Blotting | 0.5 | 1.0ug/ml |
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- AHP1592 detects a band of approximately 40kDa in human tissue spleen cell lysates.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
|
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| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
|
|
| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
|
|
| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
|
|
| Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
|
|
| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
|
|
| Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | F IF WB | 0.1 mg |
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Antigen Retrieval Buffer, pH8.0 | BUF025A | P | 500 ml | ||
| 100x Antigen Retrieval Buffer, pH8.0 | BUF025C | P | 50 ml | ||
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml |
Product Specific References
Further Reading
-
Zamzami, N. & Kroemer, G. (1999) Condensed matter in cell death.
Nature. 401 (6749): 127-8. -
Yasuda, T. et al. (1998) Molecular cloning of the cDNA encoding human deoxyribonuclease II.
J Biol Chem. 273 (5): 2610-6. -
Krieser, R.J. & Eastman, A. (1998) The cloning and expression of human deoxyribonuclease II. A possible role in apoptosis.
J Biol Chem. 273 (47): 30909-14. -
Shiokawa, D. & Tanuma, S. (1998) Cloning of cDNAs encoding porcine and human DNase II.
Biochem Biophys Res Commun. 247 (3): 864-9.
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