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DNase II antibody

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Anti Human DNase II (C-Terminal) Antibody thumbnail image 1 Anti Human DNase II (C-Terminal) Antibody thumbnail image 2
Anti Human DNase II (C-Terminal) Antibody gallery image 1
Western blot analysis of human spleen lysate probed with Rabbit anti Human DNASE-II (AHP1592) either in the absence (1) or presence (2) of blocking peptide.
Anti Human DNase II (C-Terminal) Antibody gallery image 2
Immunohistochemical staining of human spleen with Rabbit anti DNASE-II (AHP1592).

Rabbit anti Human DNase II (C-Terminal)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP1592
Datasheet
P* WB
SDS
0.1 mg loader

Rabbit anti Human DNAase II antibody recognizes human DNase II, also known as Deoxyribonuclease II. DNAaseII is an enzyme involved in both the condensation of chromatin and the fragmentation of DNA.

DNase II is ubiquitously expressed in human tissues.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to human DNase II (CT) was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
Immunogen
A peptide corresponding to amino acids near the carboxy terminus of human DNase II precursor.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml

Storage Information

Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Guarantee
12 months from date of despatch

More Information

UniProt
Q8WZ79
O00115
Entrez Gene
DNASE2
DNASE2B
GO Terms
GO:0005515 protein binding
GO:0003677 DNA binding
GO:0006915 apoptosis
GO:0005764 lysosome
GO:0004531 deoxyribonuclease II activity
GO:0006259 DNA metabolic process
Regulatory
For research purposes only

Applications of DNase II antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1 5ug/ml
Western Blotting 0.5 1.0ug/ml
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1592 detects a band of approximately 40kDa in human tissue spleen cell lysates.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA F IF WB 0.1 mg

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A P 500 ml
100x Antigen Retrieval Buffer, pH8.0 BUF025C P 50 ml
TidyBlot Western Blot Detection Reagent:HRP STAR209P WB* 0.5 ml

Product Specific References

Further Reading

  1. Zamzami, N. & Kroemer, G. (1999) Condensed matter in cell death.
    Nature. 401 (6749): 127-8.
  2. Yasuda, T. et al. (1998) Molecular cloning of the cDNA encoding human deoxyribonuclease II.
    J Biol Chem. 273 (5): 2610-6.
  3. Krieser, R.J. & Eastman, A. (1998) The cloning and expression of human deoxyribonuclease II. A possible role in apoptosis.
    J Biol Chem. 273 (47): 30909-14.
  4. Shiokawa, D. & Tanuma, S. (1998) Cloning of cDNAs encoding porcine and human DNase II.
    Biochem Biophys Res Commun. 247 (3): 864-9.

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