DKK1 antibody
Rabbit anti Human DKK1 antibody recognises human Dickkopf (Xenopus laevis) homolog 1 (DKK1), a 35 kDa to 50 kDa member of the dickkopf family. It is expressed in the placenta, prostate and to a lesser extent, in the colon and spleen. DKK1 is secreted when expressed in 293T cells.
DKK1 inhibits Wnt signalling in embryonic development, most likely acting upstream of frizzled. It is also thought to contribute to neoplastic processes, inhibit osteoblast differentiation and is associated with the presence of osteolytic bone lesions in patients with multiple myeloma.
DKK1 inhibits Wnt signalling in embryonic development, most likely acting upstream of frizzled. It is also thought to contribute to neoplastic processes, inhibit osteoblast differentiation and is associated with the presence of osteolytic bone lesions in patients with multiple myeloma.
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.1% Sodium Azide (NaN3)
- Immunogen
- Synthetic human DKK1 - KLH conjugated.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- O94907
- Entrez Gene
- DKK1
- GO Terms
- GO:0005886 plasma membrane
- GO:0002090 regulation of receptor internalization
- GO:0004871 signal transducer activity
- GO:0016055 Wnt receptor signaling pathway
- GO:0005615 extracellular space
- GO:0008083 growth factor activity
- GO:0010553 negative regulation of gene-specific transcription from RNA polymerase II promoter
- GO:0031333 negative regulation of protein complex assembly
- GO:0033137 negative regulation of peptidyl-serine phosphorylation
- GO:0048019 receptor antagonist activity
- GO:0050750 low-density lipoprotein particle receptor binding
- GO:0090082 positive regulation of heart induction by negative regulation of canonical Wnt receptor signaling pathway
- Regulatory
- For research purposes only
Applications of DKK1 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunohistology - Paraffin 1 | 10ug/ml |
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Histology Positive Control Tissue
- Uterus, endometrium.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
|
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| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
|
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| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
|
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| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
|
|
| Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
|
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| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
|
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| Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | F IF WB | 0.1 mg |
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Antigen Retrieval Buffer, pH8.0 | BUF025A | P | 500 ml | ||
| 100x Antigen Retrieval Buffer, pH8.0 | BUF025C | P | 50 ml |
Product Specific References
References for DKK1 antibody
-
Krupnik, V.E. et al. (1999) Functional and structural diversity of the human Dickkopf gene family.
Gene. 238 (2): 301-13. -
Fedi, P. et al. (1999) Isolation and biochemical characterization of the human Dkk-1 homologue, a novel inhibitor of mammalian Wnt signaling.
J Biol Chem. 274 (27): 19465-72. -
Niehrs C. (2006) Function and biological roles of the Dickkopf family of Wnt modulators.
Oncogene. 25:7469-81.
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