Rabbit anti Human DKK1 antibody recognises human Dickkopf (Xenopus laevis) homolog 1 (DKK1), a 35 kDa to 50 kDa member of the dickkopf family. It is expressed in the placenta, prostate and to a lesser extent, in the colon and spleen. DKK1 is secreted when expressed in 293T cells.
DKK1 inhibits Wnt signalling in embryonic development, most likely acting upstream of frizzled. It is also thought to contribute to neoplastic processes, inhibit osteoblast differentiation and is associated with the presence of osteolytic bone lesions in patients with multiple myeloma.
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.1% Sodium Azide (NaN3)
- Synthetic human DKK1 - KLH conjugated.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
regulation of receptor internalization
signal transducer activity
Wnt receptor signaling pathway
growth factor activity
negative regulation of gene-specific transcription from RNA polymerase II promoter
negative regulation of protein complex assembly
negative regulation of peptidyl-serine phosphorylation
receptor antagonist activity
low-density lipoprotein particle receptor binding
positive regulation of heart induction by negative regulation of canonical Wnt receptor signaling pathway
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of DKK1 antibody
|Immunohistology - Paraffin 1
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Histology Positive Control Tissue
- Uterus, endometrium.
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Secondary Antibodies Available
Useful Reagents Available
Product Specific References
References for DKK1 antibody
Krupnik, V.E. et al. (1999) Functional and structural diversity of the human Dickkopf gene family.
Gene. 238 (2): 301-13.
Fedi, P. et al. (1999) Isolation and biochemical characterization of the human Dkk-1 homologue, a novel inhibitor of mammalian Wnt signaling.
J Biol Chem. 274 (27): 19465-72.
Niehrs C. (2006) Function and biological roles of the Dickkopf family of Wnt modulators.
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