DKK1 antibody

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Rabbit anti Human DKK1

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP1156
Datasheet
P*
MSDS
50 µg loader

Rabbit anti Human DKK1 antibody recognises human Dickkopf (Xenopus laevis) homolog 1 (DKK1), a 35 kDa to 50 kDa member of the dickkopf family. It is expressed in the placenta, prostate and to a lesser extent, in the colon and spleen. DKK1 is secreted when expressed in 293T cells.

DKK1 inhibits Wnt signalling in embryonic development, most likely acting upstream of frizzled. It is also thought to contribute to neoplastic processes, inhibit osteoblast differentiation and is associated with the presence of osteolytic bone lesions in patients with multiple myeloma.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.1% Sodium Azide (NaN3)
Immunogen
Synthetic human DKK1 - KLH conjugated.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
O94907
Entrez Gene
DKK1
GO Terms
GO:0005886 plasma membrane
GO:0002090 regulation of receptor internalization
GO:0004871 signal transducer activity
GO:0016055 Wnt receptor signaling pathway
GO:0005615 extracellular space
GO:0008083 growth factor activity
GO:0010553 negative regulation of gene-specific transcription from RNA polymerase II promoter
GO:0031333 negative regulation of protein complex assembly
GO:0033137 negative regulation of peptidyl-serine phosphorylation
GO:0048019 receptor antagonist activity
GO:0050750 low-density lipoprotein particle receptor binding
GO:0090082 positive regulation of heart induction by negative regulation of canonical Wnt receptor signaling pathway
Regulatory
For research purposes only

Applications of DKK1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1 10ug/ml
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Uterus, endometrium.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA F IF WB 0.1 mg

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A P 500 ml
Antigen Retrieval Buffer, pH8.0 BUF025C P 50 ml
Histar Detection System STAR3000A C P 50 Tests

Product Specific References

References for DKK1 antibody

  1. Krupnik, V.E. et al. (1999) Functional and structural diversity of the human Dickkopf gene family.
    Gene. 238 (2): 301-13.
  2. Fedi, P. et al. (1999) Isolation and biochemical characterization of the human Dkk-1 homologue, a novel inhibitor of mammalian Wnt signaling.
    J Biol Chem. 274 (27): 19465-72.
  3. Niehrs C. (2006) Function and biological roles of the Dickkopf family of Wnt modulators.
    Oncogene. 25:7469-81.