Delta-Like Protein 4 antibody



DLL4 is expressed in the vasculature and plays a critical role in vascular development. It is induced by vascular endothelial growth factor (VEGF), as a negative feedback regulator to regulate angiogenic sprouting and promote the formation of a differentiated vascular network (Mailhos et al. 2001). DLL4 has been found to be strongly expressed in tumour vessels of primary renal tumours (Patel et al. 2005) and bladder cancer (Patel et al. 2006), and inhibition of DLL4 results in increased vascular proliferation but defective maturation. This in turn leads to a decrease in tumour growth, with no apparent toxicity (Ridgway et al. 2006).
Expression of DLL4 in normal human dermis is low in foetal tissues, becomes more intense during early life (0-20 years) and gradually declines thereafter as shown by immunohistochemical studies on FFPE tissues using rabbit anti human DLL4 antibody (Gunin et al. 2014).
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Expected from Sequence Rat Expected from Sequence Chimpanzee Expected from Sequence - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to DLL4 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Synthetic peptide corresponding to the internal region of human DLL4.
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- Q9NR61
- Entrez Gene
- DLL4
- GO Terms
- GO:0005886 plasma membrane
- GO:0005509 calcium ion binding
- GO:0016021 integral to membrane
- GO:0005112 Notch binding
- GO:0007154 cell communication
- GO:0007219 Notch signaling pathway
- GO:0007220 Notch receptor processing
- GO:0008015 blood circulation
- GO:0030154 cell differentiation
- Regulatory
- For research purposes only
Applications of Delta-Like Protein 4 antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
ELISA | 1/3000 | 1/15000 | |
Immunohistology - Paraffin | 5 | 15ug/ml | |
Western Blotting | 1/300 | 1/1500 |
- Histology Positive Control Tissue
- Human ovary.
- Western Blotting
- AHP1274 detects a band of approximately 74kDa in pancreatic cell lysates.
Secondary Antibodies Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
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Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
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Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
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Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
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Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
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Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
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Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | F IF WB | 0.1 mg | ||
Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | F IF WB | 0.1 mg | ||
Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | F IF WB | 0.1 mg |
Useful Reagents Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Antigen Retrieval Buffer, pH8.0 | BUF025A | P | 500 ml | ||
Antigen Retrieval Buffer, pH8.0 | BUF025C | P | 50 ml | ||
TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml |
Product Specific References
References for Delta-Like Protein 4 antibody
-
You, C. et al. (2013) Loss of CCM3 impairs DLL4-Notch signalling: implication in endothelial angiogenesis and in inherited cerebral cavernous malformations.
J Cell Mol Med. 17 (3): 407-18. -
Villaamil, V.M. et al. (2012) Multiple biomarker tissue arrays: A computational approach to identifying protein-protein interactions in the EGFR/ERK signalling pathway.
J Mol Signal. 7: 14. -
Gunin, A.G. et al. (2014) Age-related changes in angiogenesis in human dermis.
Exp Gerontol. 55C: 143-51. -
El Hindy, N. et al. (2013) Implications of Dll4-Notch signaling activation in primary glioblastoma multiforme.
Neuro Oncol. 15: 1366-78. -
Medina Villaamil, V. et al. (2012) Searching for Hif1-α interacting proteins in renal cell carcinoma.
Clin Transl Oncol. 14: 698-708. -
Gunin, A.G. et al. (2014) Age-related changes in angiogenesis in human dermis.
Exp Gerontol. 55: 143-51. -
Hjelmgren O et al. (2016) Increased Vascularization in the Vulnerable Upstream Regions of Both Early and Advanced Human Carotid Atherosclerosis.
PLoS One. 11 (12): e0166918.
Further Reading
-
Thurston, G. et al. (2007) The Delta paradox: DLL4 blockade leads to more tumour vessels but less tumour growth.
Nat Rev Cancer. 7 (5): 327-31.
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