Defensin Beta 3 antibody

Rabbit anti Human Defensin Beta 3

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
Defensin Beta 3

Product Code Applications Pack Size List Price Your Price Qty
AHP1802
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SDS Safety Datasheet SDS
E WB 0.1 mg loader
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Rabbit anti human Defensinβ-3 polyclonal antibody recognizes human defensin beta 3 (BD-3), a 45aa ~5 kDa cationic non-haemolytic antimicrobial peptide expressed by epithelial cells and member of the beta-defensin family. Defensin beta 3 is highly expressed in the skin and tonsils and at lower levels in other tissues(Harder et al. 2001).

Defensin beta 3 plays an important role in the innate immune response to potentially pathogenic microbes, including multiresistant S. aureus and vancomycin-resistant E. faecium.

Target Species
Human
Product Form
Purified IgG - lyophilized
Reconstitution
Reconstitute with 0.1ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
Antiserum Preparation
Antiserum to human defensin beta 3 was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Carrier Free
Yes
Approx. Protein Concentrations
IgG concentration 1.0mg/ml after reconstitution
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.

This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.5 2.0ug/ml
Western Blotting 0.1 0.2ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
ELISA
This purified human Defensinβ-3 antibody may be used in an indirect ELISA with recombinant human Defensinβ-3 (PHP211) as the standard.

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References for Defensin Beta 3 antibody

  1. Boesch, A.W. et al. (2013) A multiplexed assay to detect antimicrobial peptides in biological fluids and cell secretions.
    J Immunol Methods. 397 (1-2): 71-6.

Further Reading

  1. Harder, J. et al. (2001) Isolation and characterization of human beta -defensin-3, a novel human inducible peptide antibiotic.
    J Biol Chem. 276: 5707-13.

Synonyms
BD-3
RRID
AB_2091838
UniProt
P81534
Entrez Gene
DEFB103B
GO Terms
GO:0042742 defense response to bacterium
GO:0005576 extracellular region
GO:0006965 positive regulation of biosynthetic process of antibacterial peptides active against Gram-positive bacteria
View more products with DEFENSIN specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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