Rabbit anti Human defensinβ-1 polyclonal antibody
recognizes human defensin beta 1 (BD-1), a secreted cationic antimicrobial peptide and member of the beta-defensin family, expressed at epithelial surfaces, which plays an important role in innate immunity.
The activity of BD-1 is compromised in patients with Cystic Fibrosis due to salt-dependent inactivation (Goldman et al. 1997
- Target Species
- Product Form
- Purified IgG - lyophilised
- Reconstitute with 0.1ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
- Antiserum Preparation
- Antiserum to human defensin beta 1 was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Recombinant human defensinβ-1.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml after reconstitution
- Prior to reconstitution store at -20oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- Guaranteed for 3 months from the date of reconstitution or until the date of expiry, whichever comes first. Please see label for expiry date.
- Entrez Gene
- GO Terms
defense response to bacterium
G-protein coupled receptor protein signaling pathway
innate immune response
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of Defensin Beta 1 antibody
|Immunohistology - Paraffin 1
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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Secondary Antibodies Available
Useful Reagents Available
Application Based External Images
Product Specific References
References for Defensin Beta 1 antibody
Kelly CJ et al. (2013) Fundamental role for HIF-1α in constitutive expression of human β defensin-1.
Mucosal Immunol. 6 (6): 1110-8.
Goldman, M.J. et al. (1997) Human beta-defensin-1 is a salt-sensitive antibiotic in lung that is inactivated in cystic fibrosis.
Cell. 88: 553-60.