Defensin Beta 1 antibody


The activity of BD-1 is compromised in patients with Cystic Fibrosis due to salt-dependent inactivation (Goldman et al. 1997).
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - lyophilised
- Reconstitution
- Reconstitute with 0.1ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended. - Antiserum Preparation
- Antiserum to human defensin beta 1 was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Immunogen
- Recombinant human defensinβ-1.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml after reconstitution
Storage Information
- Storage
- Prior to reconstitution store at -20oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- Guaranteed for 3 months from the date of reconstitution or until the date of expiry, whichever comes first. Please see label for expiry date.
More Information
- UniProt
- P60022 Related reagents
- Entrez Gene
- DEFB1 Related reagents
- GO Terms
- GO:0042742 defense response to bacterium
- GO:0005576 extracellular region
- GO:0006935 chemotaxis
- GO:0007186 G-protein coupled receptor protein signaling pathway
- GO:0045087 innate immune response
- Regulatory
- For research purposes only
Applications of Defensin Beta 1 antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
ELISA | 0.5 | 2.0ug/ml | |
Immunohistology - Paraffin 1 | |||
Western Blotting | 0.1 | 0.2ug/ml |
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Secondary Antibodies Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Sheep anti Rabbit IgG:Biotin | 2AB02B | C E P WB | 1 ml |
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Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
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Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
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Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
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Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
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Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
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Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
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Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | F IF WB | 0.1 mg | ||
Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | F IF WB | 0.1 mg | ||
Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | F IF WB | 0.1 mg |
Useful Reagents Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Antigen Retrieval Buffer, pH8.0 | BUF025A | P | 500 ml | ||
Antigen Retrieval Buffer, pH8.0 | BUF025C | P | 50 ml | ||
TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml |
Product Specific References
References for Defensin Beta 1 antibody
-
Kelly CJ et al. (2013) Fundamental role for HIF-1α in constitutive expression of human β defensin-1.
Mucosal Immunol. 6 (6): 1110-8.
Further Reading
-
Goldman, M.J. et al. (1997) Human beta-defensin-1 is a salt-sensitive antibiotic in lung that is inactivated in cystic fibrosis.
Cell. 88: 553-60.