Defensin Alpha 1 antibody
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Goat anti Human Defensin Alpha 1
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- Defensin Alpha 1
Antibody Quality and 
Unrivaled 
| Goat anti Human defensin α-1 antibody recognizes defensin alpha 1, otherwise known as HNP-1 or neutrophil defensin 1. Defensin α-1 is a secreted cationic antimicrobial peptide and member of the alpha-defensin family, which plays an important role in the innate immune response against microbial infections. Synovial fluid defensin α-1 levels act as a biomarker for the evaluation of periprosthetic joint infection, a significant cause of morbidity and burden following prosthetic joint replacement (Li et al. 2017). |
- Target Species
- Human
- Product Form
- Purified IgG - lyophilized
- Reconstitution
- Reconstitute with 0.1ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
N.B. For functional studies do not add sodium azide - Antiserum Preparation
- Antiserum to human defensin-α-1 was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Carrier Free
- Yes
- Immunogen
- Recombinant human defensin-α-1
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml after reconstitution
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
0.5 | 2.0ug/ml |
| Functional Assays | |
0.4 | 0.6ug/ml |
| Western Blotting | |
0.1 | 0.2ug/ml |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- ELISA
- This purified goat anti human defensin-α-1 antibody may be used in an indirect ELISA or as the capture reagent in a sandwich ELISA with recombinant human defensin-α-1 (PHP208) as the standard.
- Western Blotting
- This purified goat anti human defensin-α-1 antibody may be used in Western Blotting applications under either reducing or non-reducing conditions with recombinant human defensin-α-1 (PHP208) as the positive control.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
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| Rabbit anti Goat IgG (Fc):HRP | STAR122P | C E WB | 1 mg |
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| Description | Rabbit anti Goat IgG (Fc):HRP | ||||||
| Rabbit anti Goat IgG (Fc):FITC | STAR122F | F | 1 mg |
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References for Defensin Alpha 1 antibody
-
Castañeda-Delgado, J.E. et al. (2014) Tuberculin skin test and interferon-gamma release assay values are associated with antimicrobial peptides expression in polymorphonuclear cells during latent tuberculous infection.
Mem Inst Oswaldo Cruz. 109: 330-4. -
Alard, J.E. et al. (2015) Recruitment of classical monocytes can be inhibited by disturbing heteromers of neutrophil HNP1 and platelet CCL5.
Sci Transl Med. 7 (317): 317ra196.
- Synonyms
- HNP-1
- RRID
- AB_2091687
- UniProt
- P59665
- Entrez Gene
- DEFA1
- GO Terms
- GO:0042742 defense response to bacterium
- GO:0050832 defense response to fungus
- GO:0009615 response to virus
- GO:0005615 extracellular space
- GO:0006935 chemotaxis
- GO:0006955 immune response
- GO:0031640 killing of cells of other organism
View more products with DEFENSIN specificity
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