DDX6 antibody

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Goat anti DDX6

Dead-box protein 6 antibody recognizes probable ATP-dependent RNA helicase DDX6, also known as ATP-dependent RNA helicase p54 DEAD box protein 6 Oncogene RCK. The antibody has been extensively validated for western blotting using whole cell lysates.

DDX6 Antibody with Control Lysate

Dead-box protein 6 antibody recognizes probable ATP-dependent RNA helicase DDX6, also known as ATP-dependent RNA helicase p54 DEAD box protein 6 Oncogene RCK. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00061 WB 100 µl
VPA00061KT WB 2 Westerns
With Lysate 400µg
Goat anti Human DDX6 antibody recognizes DDX6 or DEAD box protein 6, a 54 kDa protein and member of the DEAD box protein family. DEAD box helicases possess a conserved helicase core. This core consists of 2 domains resembling the recombination protein RecA.

The functions of ATP dependent roles in RNA processes such as pre-mRNA splicing, RNA translation, RNA synthesis and assembly of RNA complexes.

Goat anti Human DDX6 recognizes DEAD box protein 6 as a single band of 54 kDa in a range of human cell line lysates tested.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Goat polyclonal antibody purified by affinity chromatography
Preparation
20μl Goat polyclonal antibody purified by affinity chromatography
Buffer Solution
TRIS buffered saline
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin
Immunogen
Peptide with sequence C-AEYHSEPVEDEKP from the C terminus of the protein.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
P26196 Related reagents
Entrez Gene
DDX6 Related reagents
GO Terms
GO:0005515 protein binding
GO:0000932 cytoplasmic mRNA processing body
GO:0003723 RNA binding
GO:0003724 RNA helicase activity
GO:0005829 cytosol
GO:0005524 ATP binding
GO:0008026 ATP-dependent helicase activity
GO:0010494 stress granule
GO:0043928 exonucleolytic nuclear-transcribed mRNA catabolic process involved in deadenylation-dependent decay
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of DDX6 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Western Blotting
Goat anti DDX6 detects a band of approximately 54 kDa in Jurkat cell lysates.
Western Blotting
Goat anti DDX6 detects a band of approximately 54 kDa in Jurkat cell lysates.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Donkey anti Sheep/Goat IgG:HRP STAR88P 1 ml C E P WB
Donkey anti Sheep/Goat IgG:HRP STAR88P 1 ml C E P WB