DDX5 antibody

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Goat anti DDX5

DDX5 antibody recognizes probable ATP-dependent RNA helicase DDX5, also known as DEAD box protein 5 or RNA helicase p68. The antibody has been extensively validated for western blotting using whole cell lysates.

DDX5 Antibody with Control Lysate

DDX5 antibody recognizes probable ATP-dependent RNA helicase DDX5, also known as DEAD box protein 5 or RNA helicase p68. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00062 WB 100 µl
VPA00062KT WB 2 Westerns
With Lysate 400µg
Goat anti Human DDX5 antibody recognizes DDX5, otherwise known as p68 RNA helicase, a nuclear protein and member of the DEAD box protein family which exhibit RNA-dependent ATPase activity, and play important roles in RNA metabolism/ function, including pre-mRNA splicing, RNA translation, RNA synthesis and the assembly of RNP complexes.

DDX5 interacts with Smad3 and p53 to modulate transcription, and acts as a transcriptional coactivator for estrogen receptor alpha (ERalpha).

Goat anti Human DDX5 antibody detects DDX5 as a band of 69 kDa in a range of human cell lines. Cross reactivity with rodent cell lines is also observed.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Goat polyclonal antibody purified by affinity chromatography
Preparation
20μl Goat polyclonal antibody purified by affinity chromatography
Buffer Solution
TRIS buffered saline
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin
Immunogen
Synthetic peptide sequence C-PMIGYPMPTGYSQ from the C-terminal region of DDX5.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
P17844 Related reagents
Entrez Gene
DDX5 Related reagents
GO Terms
GO:0005515 protein binding
GO:0016049 cell growth
GO:0003723 RNA binding
GO:0003724 RNA helicase activity
GO:0005524 ATP binding
GO:0005681 spliceosomal complex
GO:0005730 nucleolus
GO:0006397 mRNA processing
GO:0008026 ATP-dependent helicase activity
GO:0008380 RNA splicing
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of DDX5 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Western Blotting
Goat anti DDX5 detects a band of approximately 69 kDa in Jurkat cell lysates.
Western Blotting
Goat anti DDX5 detects a band of approximately 69 kDa in Jurkat cell lysates.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Donkey anti Sheep/Goat IgG:HRP STAR88P 1 ml C E P WB
Donkey anti Sheep/Goat IgG:HRP STAR88P 1 ml C E P WB