DDX21 antibody

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Rabbit anti DDX21

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Product Code Applications Pack Size List Price Quantity
VPA00249
Datasheet
WB
MSDS
100 µl loader

Rabbit anti Human DDX21 antibody recognizes nucleolar RNA helicase 2, also known as DEAD (Asp-Glu-Ala-Asp) box polypeptide 21, DEAD box protein 21, Gu protein, RH II/Gu, RNA helicase II/Gu alpha, gu-alpha and nucleolar RNA helicase Gu.

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. Encoded by the DDX21 gene, nucleolar RNA helicase 2 is a DEAD box protein, which is an antigen recognized by autoimmune antibodies from a patient with watermelon stomach disease. This protein unwinds double-stranded RNA, folds single-stranded RNA, and may play important roles in ribosomal RNA biogenesis, RNA editing, RNA transport, and general transcription (provided by RefSeq, Jul 2008).

Rabbit anti Human DDX21 antibody detects a band of 87 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti DDX21 detects a band of approximately 87 kDa in K562 cell lysates
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
KLH conjugated synthetic peptide between 128-156 amino acids from the N-terminal region of human DDX21

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Guarantee
12 months from date of despatch

More Information

UniProt
Q9NR30
Entrez Gene
DDX21
GO Terms
GO:0005515 protein binding
GO:0003723 RNA binding
GO:0004004 ATP-dependent RNA helicase activity
GO:0005524 ATP binding
GO:0005730 nucleolus
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of DDX21 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P WB 2 ml