DDX21 antibody

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Anti DDX21 Antibody (PrecisionAb Polyclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with DDX21 antibody followed by detection with Goat anti Rabbit HRP conjugated(1/10,000,STAR208P) and visualized on the ChemiDoc MP with 60 second exposure. Arrow points to DDX21 (molecular weight 87 kDa)

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Rabbit anti DDX21

Product Type: PrecisionAb Polyclonal
Isotype: Polyclonal IgG
Format: Purified
Specificity: DDX21

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VPA00249 Datasheet Datasheet SDS SDS	WB	100 µl		Log in	Get Quote
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Rabbit anti Human DDX21 antibody recognizes nucleolar RNA helicase 2, also known as DEAD (Asp-Glu-Ala-Asp) box polypeptide 21, DEAD box protein 21, Gu protein, RH II/Gu, RNA helicase II/Gu alpha, gu-alpha and nucleolar RNA helicase Gu.

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. Encoded by the DDX21 gene, nucleolar RNA helicase 2 is a DEAD box protein, which is an antigen recognized by autoimmune antibodies from a patient with watermelon stomach disease. This protein unwinds double-stranded RNA, folds single-stranded RNA, and may play important roles in ribosomal RNA biogenesis, RNA editing, RNA transport, and general transcription (provided by RefSeq, Jul 2008).

Rabbit anti Human DDX21 antibody detects a band of 87 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Anti DDX21 detects a band of approximately 87 kDa in K562 cell lysates.
Product Form: Purified IgG - liquid
Preparation: Rabbit polyclonal antibody purified by affinity chromatography.
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃).
Immunogen: KLH conjugated synthetic peptide between 128-156 amino acids from the N-terminal region of human DDX21
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Rabbit IgG (H/L):HRP	STAR208P	WB	2 ml	Log in	Get Quote
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Description	Goat anti Rabbit IgG (H/L):HRP

UniProt: Q9NR30
Entrez Gene: DDX21
GO Terms: GO:0005515 protein binding; GO:0003723 RNA binding; GO:0004004 ATP-dependent RNA helicase activity; GO:0005524 ATP binding; GO:0005730 nucleolus