DDX17 antibody

Rabbit anti DDX17

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Specificity
DDX17

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VPA00304
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Rabbit anti Human DDX17 antibody recognizes DDX17, also known as DEAD (Asp-Glu-Ala-Asp) box polypeptide 17, DEAD box protein p72 and RNA-dependent helicase p72.

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and splicesosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The DDX17 gene encodes a DEAD box protein, which is an ATPase activated by a variety of RNA species, but not by dsDNA. DDX17 is closely related to DDX5. The DDX17 gene can encode multiple isoforms due to both alternative splicing and the use of alternative translation initiation codons, including a non-AUG (CUG) start codon (provided by RefSeq, Apr 2011).

Rabbit anti Human DDX17 antibody detects bands of 72 and 80 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti DDX17 detects bands of approximately 72 and 80 kDa in Jurkat cell lysate.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3).
Immunogen
KLH-conjugated synthetic peptide corresponding to aa 128-155 of human DDX17
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

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RRID
AB_2813828.
UniProt
Q92841
Entrez Gene
DDX17
GO Terms
GO:0003723 RNA binding
GO:0003724 RNA helicase activity
GO:0005524 ATP binding
GO:0005634 nucleus
GO:0006396 RNA processing
GO:0008026 ATP-dependent helicase activity
GO:0008186 RNA-dependent ATPase activity
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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