DDX17 antibody

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Rabbit anti DDX17

Rabbit anti human DDX17 antibody recognizes the probable ATP-dependent RNA helicase DDX17, also known as DEAD (Asp-Glu-Ala-Asp) box polypeptide 17, DEAD box protein p72, or RNA-dependent helicase p72. This antibody has been extensively validated for western blotting using whole cell lysates.

DDX17 Antibody with Control Lysate

Rabbit anti human DDX17 antibody recognizes the probable ATP-dependent RNA helicase DDX17, also known as DEAD (Asp-Glu-Ala-Asp) box polypeptide 17, DEAD box protein p72, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 17 (72kD) or RNA-dependent helicase p72.

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00304 WB 100 µl
VPA00304KT WB 2 Westerns
With Lysate 400µg
Rabbit anti Human DDX17 antibody recognizes DDX17, also known as DEAD (Asp-Glu-Ala-Asp) box polypeptide 17, DEAD box protein p72 and RNA-dependent helicase p72.

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and splicesosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The DDX17 gene encodes a DEAD box protein, which is an ATPase activated by a variety of RNA species, but not by dsDNA. DDX17 is closely related to DDX5. The DDX17 gene can encode multiple isoforms due to both alternative splicing and the use of alternative translation initiation codons, including a non-AUG (CUG) start codon (provided by RefSeq, Apr 2011).

Rabbit anti Human DDX17 antibody detects bands of 72 and 80 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti DDX17 detects bands of approximately 72 and 80 kDa in Jurkat cell lysate
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography
Preparation
20μl Rabbit polyclonal antibody purified by affinity chromatography
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
KLH-conjugated synthetic peptide corresponding to aa 128-155 of human DDX17
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg Jurkat lysate lyophilized in RIPA buffer
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch
Shelf Life
As supplied, 12 months from date of despatch

More Information

UniProt
Q92841 Related reagents
Entrez Gene
DDX17 Related reagents
GO Terms
GO:0003723 RNA binding
GO:0003724 RNA helicase activity
GO:0005524 ATP binding
GO:0005634 nucleus
GO:0006396 RNA processing
GO:0008026 ATP-dependent helicase activity
GO:0008186 RNA-dependent ATPase activity
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of DDX17 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB

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