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DDB1 antibody

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Anti DDB1 (C-Terminal) Antibody thumbnail image 1 Anti DDB1 (C-Terminal) Antibody thumbnail image 2 Anti DDB1 (C-Terminal) Antibody thumbnail image 3
Anti DDB1 (C-Terminal) Antibody gallery image 1
Western blot analysis of DDB1 in NSO cell lysate with Goat anti DDB1 antibody (AHP632)
Anti DDB1 (C-Terminal) Antibody gallery image 2
Western blotting analysis of HeLa (A), HepG2 (B) and Jurkat (C) lysate (35μg protein in RIPA buffer) probed with Goat anti Human DDB1 antibody (AHP632) at 1.0μgml-1. Primary incubation was 1 hour. Signal was detected by chemiluminescence
Anti DDB1 (C-Terminal) Antibody gallery image 3
Western blotting of NIH3T3 (A) and NSO (B) lysate. (35μg protein in RIPA buffer) probed with Goat anti Human DDB1 antibody (AHP632) at 0.01μgml-1. Primary incubation was 1 hour. Signal was detected by chemiluminescence

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WB

Goat anti DDB1 (C-Terminal)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
DDB1
Region
(C-TERMINAL)

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Product Code Applications Pack Size List Price Your Price Qty
AHP632
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
WB 0.1 mg
List Price Your Price

Goat anti DDB1 antibody recognizes human DNA damage-binding protein 1, also known as DDB p127 subunit, DNA damage-binding protein a, Damage-specific DNA-binding protein 1, HBV X-associated protein 1, UV-damaged DNA-binding protein 1, XPE-binding factor or Xeroderma pigmentosum group E-complementing protein. DDB1 is an 1140 amino acid ~ kDA cytoplasmic protein that translocated to the nucleus following UV irradiation and subsequently accumulates at the site of DNA damage wjere it forms part of the UV-DDB complex and is involved in the nucleotide-excision repair of DNA following damage caused by ultraviolet radiation.

Goat anti DDB1 antibody binds to an epitope within the C-terminal (CT) region of damage-specific DNA binding protein 1.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat Expected from Sequence
Mouse
Pig
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to DDB1 (CT) were raised by repeated immunisations of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
0.02%Sodium Azide
0.5%Bovine Serum Albumin
Immunogen
Peptide sequence DLIKVVEELTRIH corresponding to the C-terminal region of DDB1 (NP_001914).
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 0.2ug/ml 1ug/ml
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls
Western Blotting
AHP632 detects a band of approximately 130kDa in HeLa, Jurkat and NSO (mouse) cell lysates.

Description Product Code Applications Pack Size List Price Your Price Quantity
Rabbit anti Goat IgG (Fc):FITC STAR122F F 1 mg loader
List Price Your Price
loader
Description Rabbit anti Goat IgG (Fc):FITC
Rabbit anti Goat IgG (Fc):HRP STAR122P C E WB 1 mg loader
List Price Your Price
loader
Description Rabbit anti Goat IgG (Fc):HRP

References for DDB1 antibody

  1. Nichols, A.F. et al. (2000) Human damage-specific DNA-binding protein p48. Characterization of XPE mutations and regulation following UV irradiation.
    J Biol Chem. 275 (28): 21422-8.

Further Reading

  1. Dualan, R. et al. (1995) Chromosomal localization and cDNA cloning of the genes (DDB1 and DDB2) for the p127 and p48 subunits of a human damage-specific DNA binding protein.
    Genomics. 29 (1): 62-9.

RRID
AB_2088650
UniProt
Q16531
Entrez Gene
DDB1
GO Terms
GO:0000075 cell cycle checkpoint
GO:0005515 protein binding
GO:0042787 protein ubiquitination involved in ubiquitin-dependent protein catabolic process
GO:0000718 nucleotide-excision repair, DNA damage removal
GO:0003684 damaged DNA binding
GO:0005737 cytoplasm
GO:0005654 nucleoplasm
GO:0031464 Cul4A-RING ubiquitin ligase complex
GO:0031465 Cul4B-RING ubiquitin ligase complex
GO:0043161 proteasomal ubiquitin-dependent protein catabolic process
GO:0044419 interspecies interaction between organisms

AHP632

150779

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