DDB1 antibody

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Goat anti DDB1 (C-Terminal)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP632 WB 0.1 mg
Goat anti DDB1 antibody recognizes human DNA damage-binding protein 1, also known as DDB p127 subunit, DNA damage-binding protein a, Damage-specific DNA-binding protein 1, HBV X-associated protein 1, UV-damaged DNA-binding protein 1, XPE-binding factor or Xeroderma pigmentosum group E-complementing protein. DDB1 is an 1140 amino acid ~ kDA cytoplasmic protein that translocated to the nucleus following UV irradiation and subsequently accumulates at the site of DNA damage wjere it forms part of the UV-DDB complex and is involved in the nucleotide-excision repair of DNA following damage caused by ultraviolet radiation.

Goat anti DDB1 antibody binds to an epitope within the C-terminal (CT) region of damage-specific DNA binding protein 1.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat Expected from Sequence
Mouse
Pig
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to DDB1 (CT) were raised by repeated immunisations of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
0.02%Sodium Azide
0.5%Bovine Serum Albumin
Immunogen
Peptide sequence DLIKVVEELTRIH corresponding to the C-terminal region of DDB1 (NP_001914).
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
Q16531 Related reagents
Entrez Gene
DDB1 Related reagents
GO Terms
GO:0000075 cell cycle checkpoint
GO:0005515 protein binding
GO:0042787 protein ubiquitination involved in ubiquitin-dependent protein catabolic process
GO:0000718 nucleotide-excision repair, DNA damage removal
GO:0003684 damaged DNA binding
GO:0005737 cytoplasm
GO:0005654 nucleoplasm
GO:0031464 Cul4A-RING ubiquitin ligase complex
GO:0031465 Cul4B-RING ubiquitin ligase complex
GO:0043161 proteasomal ubiquitin-dependent protein catabolic process
GO:0044419 interspecies interaction between organisms
Regulatory
For research purposes only

Applications of DDB1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 0.2ug/ml 1ug/ml
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls
Western Blotting
AHP632 detects a band of approximately 130kDa in HeLa, Jurkat and NSO (mouse) cell lysates.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Rabbit anti Goat IgG (Fc):FITC STAR122F 1 mg F
Rabbit anti Goat IgG (Fc):HRP STAR122P 1 mg C E WB

Product Specific References

References for DDB1 antibody

  1. Nichols, A.F. et al. (2000) Human damage-specific DNA-binding protein p48. Characterization of XPE mutations and regulation following UV irradiation.
    J Biol Chem. 275 (28): 21422-8.

Further Reading

  1. Dualan, R. et al. (1995) Chromosomal localization and cDNA cloning of the genes (DDB1 and DDB2) for the p127 and p48 subunits of a human damage-specific DNA binding protein.
    Genomics. 29 (1): 62-9.