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DCTN1 antibody

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Western Blot Validated
Anti DCTN1 Antibody (PrecisionAb Polyclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with DCTN1 antibody followed by detection with HRP conjugated Donkey anti Sheep/Goat IgG (1/10,000, STAR88P) and visualized on the ChemiDoc MP with 77 second exposure. Arrow points to DCTN1 (molecular weight 150 kDa)

Goat anti DCTN1

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
PrecisionAb Antibodies
Product Code Applications Pack Size List Price Quantity
VPA00065
Datasheet
WB
MSDS
100 µl

Goat anti Human DCTN1 antibody recognizes DCTN1, the largest component of the dynactin complex. The dynactin complex, via DCTN1, regulates the microtubule-based molecular motor complex, dynein. Through this interaction DCTN1 plays a role in ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis.

Goat anti Human DCTN1 antibody detects DCTN1 as a band of 150 kDa in a wide rangle of human cell lysates. Cross reactivity with rodent cell lines is also observed.

Product Details

Target Species
Human
Western Blotting
Goat anti DCTN1 detects a band of approximately 150 kDa in Jurkat cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Goat polyclonal antibody purified by affinity chromatography
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin
Immunogen
Synthetic peptide sequence C-QEQLHQLHSRLI from the C-terminal region of DCTN1.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Guarantee
As supplied, 12 months from date of despatch.

More Information

UniProt
Q14203
Entrez Gene
DCTN1
GO Terms
GO:0000086 G2/M transition of mitotic cell cycle
GO:0005515 protein binding
GO:0000776 kinetochore
GO:0000922 spindle pole
GO:0003774 motor activity
GO:0005829 cytosol
GO:0005874 microtubule
GO:0007067 mitosis
GO:0007399 nervous system development
GO:0008219 cell death
GO:0030286 dynein complex
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of DCTN1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Donkey anti Sheep/Goat IgG:HRP STAR88P C E P WB 1 ml loader

A Precise Solution to Reproducible Western Blots - PrecisionAb Antibodies
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