DCTN1 antibody
Goat anti DCTN1
DCTN1 antibody recognizes Dynactin subunit 1, also known as 150 kDa dynein-associated polypeptide, DAP-150, DP-150 p135 or p150-glued. The antibody has been extensively validated for western blotting using whole cell lysates.
DCTN1 Antibody with Control Lysate
DCTN1 antibody recognizes Dynactin subunit 1, also known as 150 kDa dynein-associated polypeptide, DAP-150, DP-150 p135 or p150-glued. The antibody has been extensively validated for western blotting using whole cell lysates.
- Product Type
- PrecisionAb™ Polyclonal
- Isotype
- Polyclonal IgG
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VPA00065 | WB | 100 µl | ||||
| VPA00065KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Goat anti Human DCTN1 antibody recognizes DCTN1, the largest component of the dynactin complex. The dynactin complex, via DCTN1, regulates the microtubule-based molecular motor complex, dynein. Through this interaction DCTN1 plays a role in ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis.
Goat anti Human DCTN1 antibody detects DCTN1 as a band of 150 kDa in a wide rangle of human cell lysates. Cross reactivity with rodent cell lines is also observed.
Goat anti Human DCTN1 antibody detects DCTN1 as a band of 150 kDa in a wide rangle of human cell lysates. Cross reactivity with rodent cell lines is also observed.
Product Details
- Target Species
- Human
- Western Blotting
- Goat anti DCTN1 detects a band of approximately 150 kDa in Jurkat cell lysates.
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Goat polyclonal antibody purified by affinity chromatography
- Preparation
- 20μl Goat polyclonal antibody purified by affinity chromatography
- Buffer Solution
- TRIS buffered saline
- Buffer Solution
- TRIS buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin - Preservative Stabilisers
- 0.02% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin - Immunogen
- Synthetic peptide sequence C-QEQLHQLHSRLI from the C-terminal region of DCTN1.
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg Jurkat lysate lyophilized in RIPA buffer.
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch.
- Shelf Life
- As supplied, 12 months from date of despatch.
More Information
- UniProt
- Q14203 Related reagents
- Entrez Gene
- DCTN1 Related reagents
- GO Terms
- GO:0000086 G2/M transition of mitotic cell cycle
- GO:0005515 protein binding
- GO:0000776 kinetochore
- GO:0000922 spindle pole
- GO:0003774 motor activity
- GO:0005829 cytosol
- GO:0005874 microtubule
- GO:0007067 mitosis
- GO:0007399 nervous system development
- GO:0008219 cell death
- GO:0030286 dynein complex
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of DCTN1 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Donkey anti Sheep/Goat IgG:HRP | STAR88P | 1 ml | C E P WB | ||
| Donkey anti Sheep/Goat IgG:HRP | STAR88P | 1 ml | C E P WB |
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