DAXX antibody

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Rabbit anti Human DAXX

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP1421 E P* WB 50 µg
Rabbit anti Human DAXX antibody recognises human Death Domain-Associated Protein 6, a novel Fas binding protein that has been designated DAXX.

DAXX binds specifically to the death domain of Fas and enhances Fas induced apoptosis and activates the Jun N-terminal kinase (JNK) pathway.

DAXX is predominantly a nuclear protein, where it acts as a repressor of EtsI and Pax3 transactivation. When in association with ASK1 kinase DAXX translocates from the nucleus to the cytoplasm.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to human DAXX was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Sodium Azide (NaN3)
Immunogen
Synthetic peptide corresponding to amino acids 261-274 of human DAXX protein.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
Q9UER7 Related reagents
Entrez Gene
DAXX Related reagents
GO Terms
GO:0000775 chromosome, centromeric region
GO:0002039 p53 binding
GO:0006915 apoptosis
GO:0005057 receptor signaling protein activity
GO:0005730 nucleolus
GO:0006350 transcription
GO:0007257 activation of JUN kinase activity
GO:0008134 transcription factor binding
GO:0008625 induction of apoptosis via death domain receptors
GO:0016481 negative regulation of transcription
GO:0016605 PML body
GO:0031072 heat shock protein binding
GO:0030521 androgen receptor signaling pathway
GO:0031396 regulation of protein ubiquitination
GO:0031625 ubiquitin protein ligase binding
GO:0042803 protein homodimerization activity
GO:0044419 interspecies interaction between organisms
GO:0045892 negative regulation of transcription, DNA-dependent
GO:0047485 protein N-terminus binding
GO:0050681 androgen receptor binding
Regulatory
For research purposes only

Applications of DAXX antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/7000 1/45000
Immunohistology - Paraffin 1 2.5ug/ml
Western Blotting 1/200 1/1000
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Human kidney

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A 500 ml P
Antigen Retrieval Buffer, pH8.0 BUF025C 50 ml P
TidyBlot™ Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*
Histar Detection System STAR3000B 150 Tests C P