Darpp-32 antibody

Rabbit anti Darpp-32

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Specificity
Darpp-32

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VPA00687
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Rabbit anti Human DARPP-32 antibody recognizes the protein phosphatase 1 regulatory subunit 1B also known as dopamine and cAMP-regulated neuronal phosphoprotein 32.

The PPP1R1B gene encodes a bifunctional signal transduction molecule. Dopaminergic and glutamatergic receptor stimulation regulates its phosphorylation and function as a kinase or phosphatase inhibitor. As a target for dopamine, PPP1R1B may serve as a therapeutic target for neurologic and psychiatric disorders. Multiple transcript variants encoding different isoforms have been found for PPP1R1B (provided by RefSeq, Oct 2011).

Rabbit anti Human DARPP-32 antibody detects a band of 32 kDa. The antibody has been extensively validated for western blotting using mouse brain tissue lysate.
PrecisionAb Antibodies

Target Species
Human
Western Blotting
Rabbit anti DARPP-32 detects a band of approximately 32 kDa in mouse brain tissue.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography on Protein A.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide.
Immunogen
KLH conjugated synthetic peptide encompassing part of human DARPP-32 (amino acids 106 - 139)
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

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UniProt
Q9UD71
Entrez Gene
PPP1R1B
GO Terms
GO:0005829 cytosol
GO:0004860 protein kinase inhibitor activity
GO:0004864 protein phosphatase inhibitor activity
GO:0007165 signal transduction
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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