Darpp-32 antibody
Rabbit anti Human DARPP-32 antibody recognizes the protein phosphatase 1 regulatory subunit 1B also known as dopamine and cAMP-regulated neuronal phosphoprotein 32.
The PPP1R1B gene encodes a bifunctional signal transduction molecule. Dopaminergic and glutamatergic receptor stimulation regulates its phosphorylation and function as a kinase or phosphatase inhibitor. As a target for dopamine, PPP1R1B may serve as a therapeutic target for neurologic and psychiatric disorders. Multiple transcript variants encoding different isoforms have been found for PPP1R1B (provided by RefSeq, Oct 2011).
Rabbit anti Human DARPP-32 antibody detects a band of 32 kDa. The antibody has been extensively validated for western blotting using mouse brain tissue lysate.
The PPP1R1B gene encodes a bifunctional signal transduction molecule. Dopaminergic and glutamatergic receptor stimulation regulates its phosphorylation and function as a kinase or phosphatase inhibitor. As a target for dopamine, PPP1R1B may serve as a therapeutic target for neurologic and psychiatric disorders. Multiple transcript variants encoding different isoforms have been found for PPP1R1B (provided by RefSeq, Oct 2011).
Rabbit anti Human DARPP-32 antibody detects a band of 32 kDa. The antibody has been extensively validated for western blotting using mouse brain tissue lysate.
Product Details
- Target Species
- Human
- Western Blotting
- Rabbit anti DARPP-32 detects a band of approximately 32 kDa in mouse brain tissue
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Rabbit polyclonal antibody purified by affinity chromatography on Protein A
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide
- Immunogen
- KLH conjugated synthetic peptide encompassing part of human DARPP-32 (amino acids 106 - 139)
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Shelf Life
- As supplied, 12 months from date of despatch.
More Information
- UniProt
- Q9UD71 Related reagents
- Entrez Gene
- PPP1R1B Related reagents
- GO Terms
- GO:0005829 cytosol
- GO:0004860 protein kinase inhibitor activity
- GO:0004864 protein phosphatase inhibitor activity
- GO:0007165 signal transduction
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of Darpp-32 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
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Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB |
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