DAI antibody

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Rabbit anti DAI

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP1780T E P* WB* 50 µg
AHP1780 E P* WB* 0.1 mg
Rabbit anti DAI antibody recognizes human DAI, also known as Z-DNA Binding Protein (ZBP1), a ~46 kDa DNA binding protein belonging to a family of proteins that contain a Za domain which specifically binds to the left-handed Z-DNA and Z-RNA.

DAI may be involved in processes such host response to neoplasia. cytosolic DAI can act like the toll-like receptor TLR9 by detecting cytosolic double-stranded (ds) DNA and trigger induction of type I interferon and other innate immune responses. It is thought that the binding of DAI to dsDNA enhances its association with innate immune response proteins such as the IRF3 transcription factor and the serine/threonine kinase TBK1 (also known as NAK).

DAI is highly expressed in lymphatic tissues such as lymph nodes, leukocytes, tonsil, bone marrow and spleen. Expression at a lower level is also seen in thymus, lung and liver. Multiple isoforms of DAI exist.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to human DAI were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
Immunogen
Synthetic peptide corresponding to an 18 amino acid sequence from near the carboxy terminus of human ZBP1.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
Q9H171 Related reagents
Entrez Gene
ZBP1 Related reagents
GO Terms
GO:0003692 left-handed Z-DNA binding
GO:0003723 RNA binding
GO:0003726 double-stranded RNA adenosine deaminase activity
Regulatory
For research purposes only

Applications of DAI antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Immunohistology - Paraffin 1
Western Blotting 2 0.5 1.0ug/ml
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
  2. 2 Reduced and denatured conditions
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1780 detects a band of approximately 46 kDa in mouse small intestine tissue lysate

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A 500 ml P
Antigen Retrieval Buffer, pH8.0 BUF025C 50 ml P
Histar Detection System STAR3000A 50 Tests C P

Product Specific References

References for DAI antibody

  1. Takaoka, A. et al. (2007) DAI (DLM-1/ZBP1) is a cytosolic DNA sensor and an activator of innate immune response.
    Nature. 448 (7152): 501-5.
  2. Schwartz, T. et al. (2001) Structure of the DLM-1-Z-DNA complex reveals a conserved family of Z-DNA-binding proteins.
    Nat Struct Biol. 8 (9): 761-5.