Rabbit anti Human cytochrome p450 3A4 antibody recognizes human cytochrome p450 3A4 (CYP3A4). P450 enzymes are divided into two groups: steroidogenic and xenobiotic. The latter group is comprised of three families 1, 2 and 3. The xenobiotic p450's are involved in most oxidative drug metabolism. Work in this area is ongoing but studies suggest that all known drug metabolism is mediated by family members:CYP3A, CYP2D6, CYP1A2, CYP2C9/10, CYP2C19 and CYP2E1. Rabbit anti Human cytochrome p450 3A4 antibody is a neutralizing antibody, a specific and potent inhibitor of CYP3A4.
- Target Species
- Product Form
- Serum - liquid
- Antiserum Preparation
- Antisera to human CYP3A4 were raised by repeated immunisation of rabbits with highly purified antigen.
- Preservative Stabilisers
- None Present
- Purified human CYP3A4 expressed in E.Coli
- Store at -20oC. Ship at +4oC.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 18 months from date of despatch.
- Entrez Gene
- GO Terms
electron carrier activity
integral to membrane
endoplasmic reticulum membrane
steroid catabolic process
xenobiotic metabolic process
androgen metabolic process
alkaloid catabolic process
monoterpenoid metabolic process
oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen
vitamin D3 25-hydroxylase activity
taurochenodeoxycholate 6alpha-hydroxylase activity
caffeine oxidase activity
vitamin D metabolic process
exogenous drug catabolic process
heterocycle metabolic process
albendazole monooxygenase activity
quinine 3-monooxygenase activity
testosterone 6-beta-hydroxylase activity
vitamin D 24-hydroxylase activity
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of Cytochrome P450 3A4 antibody
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
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Secondary Antibodies Available
Useful Reagents Available
Application Based External Images
Product Specific References
References for Cytochrome P450 3A4 antibody
Vranová, I. et al. (2013) The Anticancer Drug Ellipticine Induces Cytochromes P450 1A1, 1A2 and 3A, Cytochrome b5 and NADPH:Cytochrome P450 Oxidoreductase in Rat Liver, Kidney and Lung
Int. J. Electrochem. Sci., 8 (2013) 1586 - 97
Ng, P.S. et al. (2003) Production of inhibitory polyclonal antibodies against cytochrome P450s.
Drug Metab Pharmacokinet. 18 (3): 163-72.
Song, Z. et al. (2009) Efficient generation of hepatocyte-like cells from human induced pluripotent stem cells.
Cell Res. 19: 1233-42.
Schmidt, A. et al. (2011) Differential expression of glutamine synthetase and cytochrome P450 isoforms in human hepatoblastoma.
Toxicology. 281 (1-3): 7-14.
Hřebačková, J. et al. (2009) Histone deacetylase inhibitors valproate and trichostatin A are toxic to neuroblastoma cells and modulate cytochrome P450 1A1, 1B1 and 3A4 expression in these cells.
Interdiscip Toxicol. 2: 205-10.
Poljaková, J. et al. (2013) Electrochemical Determination of Enzymes Metabolizing Ellipticine in Thyroid Cancer Cells - a Tool to Explain the Mechanism of Ellipticine Toxicity to these Cells
Int. J. Electrochem. Sci. 8: 1573 - 85
Strick-Marchand, H. et al. (2015) A novel mouse model for stable engraftment of a human immune system and human hepatocytes.
PLoS One. 10 (3): e0119820.
Werck-Reichhart, D. & Feyereisen, R. (2000) Cytochromes P450: a success story.
Genome Biol. 1 (6): REVIEWS3003.