Cytochrome P450 3A4 antibody

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Rabbit anti Human Cytochrome P450 3A4

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP622Z
Datasheet
FN WB
MSDS
50 µl loader

Rabbit anti Human cytochrome p450 3A4 antibody recognizes human cytochrome p450 3A4 (CYP3A4). P450 enzymes are divided into two groups: steroidogenic and xenobiotic. The latter group is comprised of three families 1, 2 and 3. The xenobiotic p450's are involved in most oxidative drug metabolism. Work in this area is ongoing but studies suggest that all known drug metabolism is mediated by family members:CYP3A, CYP2D6, CYP1A2, CYP2C9/10, CYP2C19 and CYP2E1. Rabbit anti Human cytochrome p450 3A4 antibody is a neutralizing antibody, a specific and potent inhibitor of CYP3A4.

Product Details

Target Species
Human
Product Form
Serum - liquid
Antiserum Preparation
Antisera to human CYP3A4 were raised by repeated immunisation of rabbits with highly purified antigen.
Preservative Stabilisers
None Present
Immunogen
Purified human CYP3A4 expressed in E.Coli

Storage Information

Storage
Store at -20oC. Ship at +4oC.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
18 months from date of despatch.

More Information

UniProt
P08684 Related reagents
Entrez Gene
CYP3A4 Related reagents
GO Terms
GO:0009055 electron carrier activity
GO:0016021 integral to membrane
GO:0005496 steroid binding
GO:0005789 endoplasmic reticulum membrane
GO:0005792 microsome
GO:0006706 steroid catabolic process
GO:0006805 xenobiotic metabolic process
GO:0008209 androgen metabolic process
GO:0009822 alkaloid catabolic process
GO:0009986 cell surface
GO:0019825 oxygen binding
GO:0016098 monoterpenoid metabolic process
GO:0016712 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen
GO:0019899 enzyme binding
GO:0020037 heme binding
GO:0030343 vitamin D3 25-hydroxylase activity
GO:0033780 taurochenodeoxycholate 6alpha-hydroxylase activity
GO:0034875 caffeine oxidase activity
GO:0042359 vitamin D metabolic process
GO:0042738 exogenous drug catabolic process
GO:0046483 heterocycle metabolic process
GO:0047638 albendazole monooxygenase activity
GO:0050591 quinine 3-monooxygenase activity
GO:0050649 testosterone 6-beta-hydroxylase activity
GO:0070576 vitamin D 24-hydroxylase activity
GO:0070989 oxidative demethylation
Regulatory
For research purposes only

Applications of Cytochrome P450 3A4 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Functional Assays 2-20ul
Western Blotting 1/5000 1/10000
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B C E P WB 1 ml loader
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA F IF WB 0.1 mg

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
TidyBlot Western Blot Detection Reagent:HRP STAR209P WB* 0.5 ml

Application Based External Images

Immunofluorescence

Product Specific References

References for Cytochrome P450 3A4 antibody

  1. Vranová, I. et al. (2013) The Anticancer Drug Ellipticine Induces Cytochromes P450 1A1, 1A2 and 3A, Cytochrome b5 and NADPH:Cytochrome P450 Oxidoreductase in Rat Liver, Kidney and Lung
    Int. J. Electrochem. Sci., 8 (2013) 1586 - 97
  2. Ng, P.S. et al. (2003) Production of inhibitory polyclonal antibodies against cytochrome P450s.
    Drug Metab Pharmacokinet. 18 (3): 163-72.
  3. Song, Z. et al. (2009) Efficient generation of hepatocyte-like cells from human induced pluripotent stem cells.
    Cell Res. 19: 1233-42.
  4. Schmidt, A. et al. (2011) Differential expression of glutamine synthetase and cytochrome P450 isoforms in human hepatoblastoma.
    Toxicology. 281 (1-3): 7-14.
  5. Hřebačková, J. et al. (2009) Histone deacetylase inhibitors valproate and trichostatin A are toxic to neuroblastoma cells and modulate cytochrome P450 1A1, 1B1 and 3A4 expression in these cells.
    Interdiscip Toxicol. 2: 205-10.
  6. Poljaková, J. et al. (2013) Electrochemical Determination of Enzymes Metabolizing Ellipticine in Thyroid Cancer Cells - a Tool to Explain the Mechanism of Ellipticine Toxicity to these Cells
    Int. J. Electrochem. Sci. 8: 1573 - 85
  7. Strick-Marchand, H. et al. (2015) A novel mouse model for stable engraftment of a human immune system and human hepatocytes.
    PLoS One. 10 (3): e0119820.

Further Reading

  1. Werck-Reichhart, D. & Feyereisen, R. (2000) Cytochromes P450: a success story.
    Genome Biol. 1 (6): REVIEWS3003.