Cytochrome P450 2C9 antibody

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Rabbit anti Human Cytochrome P450 2C9

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP617Z C FN WB 50 µl
Rabbit anti Human Cytochrome P450 2C9 antibody recognizes human cytochrome p450 2C9 (CYP2C9). P450 enzymes are divided into two groups: steroidogenic and xenobiotic (Hornsby et al. 1989). The latter group is comprised of three families 1, 2 and 3. The xenobiotic p450's are involved in most oxidative drug metabolism (Murray et al. 1992). Work in this area is ongoing but studies suggest that all known drug metabolism is mediated by family members:CYP3A, CYP2D6, CYP1A2, CYP2C9/10, CYP2C19 and CYP2E1.

Rabbit anti human cytochrome P450 2C9 is a neutralizing antibody which is a potent inhibitor of CYP2C9 activity. Inhibition of CYP2C19 activity has also been observed.

Product Details

Target Species
Human
Product Form
Serum - liquid
Antiserum Preparation
Antisera to human CYP2C9 were raised by repeated immunisation of rabbits with highly purified antigen.
Preservative Stabilisers
None Present
Immunogen
Purified human CYP2C9 expressed in E.coli.

Storage Information

Storage
Store at -20oC. Ship at +4oC.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P11712 Related reagents
Entrez Gene
CYP2C9 Related reagents
GO Terms
GO:0009055 electron carrier activity
GO:0005789 endoplasmic reticulum membrane
GO:0005792 microsome
GO:0006805 xenobiotic metabolic process
GO:0008144 drug binding
GO:0008202 steroid metabolic process
GO:0008395 steroid hydroxylase activity
GO:0019825 oxygen binding
GO:0016098 monoterpenoid metabolic process
GO:0018675 (S)-limonene 6-monooxygenase activity
GO:0018676 (S)-limonene 7-monooxygenase activity
GO:0019627 urea metabolic process
GO:0020037 heme binding
GO:0032787 monocarboxylic acid metabolic process
GO:0033767 4-hydroxyacetophenone monooxygenase activity
GO:0034875 caffeine oxidase activity
GO:0042738 exogenous drug catabolic process
GO:0070989 oxidative demethylation
Regulatory
For research purposes only

Applications of Cytochrome P450 2C9 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Functional Assays 2-10ul
Immunohistology - Frozen
Western Blotting 1/5000 1/10000
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
TidyBlot™ Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*

Application Based External Images

Immunohistology - Frozen

Product Specific References

References for Cytochrome P450 2C9 antibody

  1. Ng, P.S. et al. (2003) Production of inhibitory polyclonal antibodies against cytochrome P450s.
    Drug Metab Pharmacokinet. 18 (3): 163-72.
  2. Larsen, B.T. et al. (2006) Epoxyeicosatrienoic and dihydroxyeicosatrienoic acids dilate human coronary arterioles via BK(Ca) channels: implications for soluble epoxide hydrolase inhibition.
    Am J Physiol Heart Circ Physiol. 290(2): H491-9.
  3. Dai, D.P. et al. (2013) In vitro functional characterization of 37 CYP2C9 allelic isoforms found in Chinese Han population.
    Acta Pharmacol Sin. 34 (11): 1449-56.
  4. Schmelzle, M. et al. (2011) Esophageal cancer proliferation is mediated by cytochrome P450 2C9 (CYP2C9).
    Prostaglandins Other Lipid Mediat. 94 (1-2): 25-33.
  5. Guo, Y. et al. (2005) Role of CYP2C9 and its variants (CYP2C9*3 and CYP2C9*13) in the metabolism of lornoxicam in humans.
    Drug Metab Dispos. 33: 749-53.
  6. Dai, D.P. et al. (2014) CYP2C9 polymorphism analysis in Han Chinese populations: building the largest allele frequency database.
    Pharmacogenomics J. 14 (1): 85-92.
  7. Schmidt, A. et al. (2011) Differential expression of glutamine synthetase and cytochrome P450 isoforms in human hepatoblastoma.
    Toxicology. 281: 7-14.
  8. Malaplate-Armand, C. et al. (2005) Down-regulation of astroglial CYP2C, glucocorticoid receptor and constitutive androstane receptor genes in response to cocaine in human U373 MG astrocytoma cells.
    Toxicol Lett. 159: 203-11.
  9. Guo, Y. et al. (2005) Catalytic activities of human cytochrome P450 2C9*1, 2C9*3 and 2C9*13.
    Xenobiotica. 35: 853-61.
  10. Strick-Marchand, H. et al. (2015) A novel mouse model for stable engraftment of a human immune system and human hepatocytes.
    PLoS One. 10 (3): e0119820.
  11. Dai, D.P. et al. (2015) Identification and Functional Assessment of a New CYP2C9 Allelic Variant CYP2C9*59.
    Drug Metab Dispos. 43 (8): 1246-9.

Further Reading

  1. Werck-Reichhart, D. and Feyereisen, R. (2000) Protein family review Cytochromes P450: a success story.
    Genome Biology. 1: 1-9