Cytochrome P450 1A2 antibody

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Rabbit anti Human Cytochrome P450 1A2

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP610Z FN WB 50 µl
Rabbit anti Human cytochrome P450 1A2 antibody recognizes human cytochrome p450 1A2 (CYP1A2). P450 enzymes are divided into two groups: steroidogenic and xenobiotic. The latter group is comprised of three families 1, 2 and 3. The xenobiotic p450's are involved in most oxidative drug metabolism. Drug metabolism is mediated by family members:CYP3A, CYP2D6, CYP1A2, CYP2C9/10, CYP2C19 and CYP2E1. Rabbit anti Human cytochrome P450 1A2 antibody (AHP610Z) is a neutralizing antibody which is a specific and potent inhibitor of CYP1A2 activity.

Product Details

Target Species
Human
Product Form
Serum - liquid
Antiserum Preparation
Antisera to human CYP1A2 were raised by repeated immunisation of rabbits with highly purified antigen.
Preservative Stabilisers
None Present
Immunogen
Purified human CYP1A2 expressed in E.coli.

Storage Information

Storage
Store at -20oC. Ship at +4oC

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P05177 Related reagents
Entrez Gene
CYP1A2 Related reagents
GO Terms
GO:0009055 electron carrier activity
GO:0005789 endoplasmic reticulum membrane
GO:0005792 microsome
GO:0006706 steroid catabolic process
GO:0006805 xenobiotic metabolic process
GO:0009403 toxin biosynthetic process
GO:0009820 alkaloid metabolic process
GO:0019825 oxygen binding
GO:0016098 monoterpenoid metabolic process
GO:0019899 enzyme binding
GO:0020037 heme binding
GO:0032259 methylation
GO:0032451 demethylase activity
GO:0032787 monocarboxylic acid metabolic process
GO:0034875 caffeine oxidase activity
GO:0042738 exogenous drug catabolic process
GO:0070330 aromatase activity
GO:0070989 oxidative demethylation
GO:0071615 oxidative deethylation
Regulatory
For research purposes only

Applications of Cytochrome P450 1A2 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Functional Assays 1-20ul
Western Blotting 1/5000 1/10000
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
TidyBlot™ Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*

Product Specific References

References for Cytochrome P450 1A2 antibody

  1. Werck-Reichhart, D. and Feyereisen, R. (2000) Protein family review Cytochromes P450: a success story.
    Genome Biology. 1: 1-9
  2. Ng, P.S. et al. (2003) Production of inhibitory polyclonal antibodies against cytochrome P450s.
    Drug Metab Pharmacokinet. 18 (3): 163-72.