Cytochrome B245 antibody

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Goat anti Cytochrome B245

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Product Code Applications Pack Size List Price Quantity
VPA00132
Datasheet
WB
MSDS
100 µl

Goat anti Human cytochrome B245 antibody recognizes the cytochrome B245 heavy chain, also known as CGD91-phox, cytochrome b558 subunit beta, heme-binding membrane glycoprotein gp91phox, NADPH oxidase 2, neutrophil cytochrome b 91 kDa polypeptide, superoxide-generating NADPH oxidase heavy chain subunit, gp91-1, gp91-phox and p22 phagocyte B-cytochrome.

Cytochrome B245 is composed of cytochrome B alpha (CYBA) and beta (CYBB) chains. It has been proposed as a primary component of the microbicidal oxidase system of phagocytes. CYBB deficiency is one of five described biochemical defects associated with chronic granulomatous disease (CGD). In this disorder, there is decreased activity of phagocyte NADPH oxidase; neutrophils are able to phagocytize bacteria but cannot kill them in the phagocytic vacuoles. The cause of the killing defect is an inability to increase the cell's respiration and consequent failure to deliver activated oxygen into the phagocytic vacuole (provided by RefSeq, Jul 2008).

Goat anti Human cytochrome B245 antibody detects a band of 80 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti cytochrome B245 detects a band of approximately 80 kDa in HeLa cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Goat polyclonal antibody purified by affinity chromatography.
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
0.02% Sodium Azide (NaN3) 0.5 % BSA
Immunogen
Peptide with the sequence C-SYLNFARKRIKNP, from the internal region of the protein sequence.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Guarantee
As supplied, 12 months from date of despatch.

More Information

UniProt
P04839
Entrez Gene
CYBB
GO Terms
GO:0009055 electron carrier activity
GO:0005244 voltage-gated ion channel activity
GO:0022900 electron transport chain
GO:0006954 inflammatory response
GO:0016175 superoxide-generating NADPH oxidase activity
GO:0020037 heme binding
GO:0042554 superoxide anion generation
GO:0043020 NADPH oxidase complex
GO:0045087 innate immune response
GO:0045730 respiratory burst
GO:0046982 protein heterodimerization activity
GO:0050660 flavin adenine dinucleotide binding
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of Cytochrome B245 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Donkey anti Sheep/Goat IgG:HRP STAR88P C E P WB 1 ml loader