Cytochrome B245 antibody

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Anti Cytochrome B245 Antibody (PrecisionAb Polyclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with Goat anti Human cytochrome B245 antibody (**VPA00132**) followed by detection with HRP conjugated Donkey anti Sheep/Goat IgG (1/10,000, STAR88P) and visualized on the ChemiDoc MP with 24.78 second exposure. Arrow points to cytochrome B245 (molecular weight 80 kDa).

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Goat anti Cytochrome B245

Product Type: PrecisionAb Polyclonal
Isotype: Polyclonal IgG
Format: Purified
Specificity: Cytochrome B245

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References (1)
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Goat anti Human cytochrome B245 antibody recognizes the cytochrome B245 heavy chain, also known as CGD91-phox, cytochrome b558 subunit beta, heme-binding membrane glycoprotein gp91phox, NADPH oxidase 2, neutrophil cytochrome b 91 kDa polypeptide, superoxide-generating NADPH oxidase heavy chain subunit, gp91-1, gp91-phox and p22 phagocyte B-cytochrome.

Cytochrome B245 is composed of cytochrome B alpha (CYBA) and beta (CYBB) chains. It has been proposed as a primary component of the microbicidal oxidase system of phagocytes. CYBB deficiency is one of five described biochemical defects associated with chronic granulomatous disease (CGD). In this disorder, there is decreased activity of phagocyte NADPH oxidase; neutrophils are able to phagocytize bacteria but cannot kill them in the phagocytic vacuoles. The cause of the killing defect is an inability to increase the cell's respiration and consequent failure to deliver activated oxygen into the phagocytic vacuole (provided by RefSeq, Jul 2008).

Goat anti Human cytochrome B245 antibody detects a band of 80 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Anti cytochrome B245 detects a band of approximately 80 kDa in HeLa cell lysates.
Species Cross-Reactivity: Target Species Cross Reactivity

Rat; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG - liquid
Preparation: Goat polyclonal antibody purified by affinity chromatography.
Buffer Solution: TRIS buffered saline.
Preservative Stabilisers: 0.02% Sodium Azide (NaN₃) 0.5 % BSA
Immunogen: Peptide with the sequence C-SYLNFARKRIKNP, from the internal region of the protein sequence.
Regulatory: For research purposes only.
Guarantee: 12 Months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Target Species	Cross Reactivity
Rat

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting			1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

References for Cytochrome B245 antibody

Waghela, B.N. et al. (2021) Upregulation of NOX-2 and Nrf-2 Promotes 5-Fluorouracil Resistance of Human Colon Carcinoma (HCT-116) Cells.
Biochemistry (Mosc). 86 (3): 262-74.

Western Blotting

Synonyms: NOX2
UniProt: P04839
Entrez Gene: CYBB
GO Terms: GO:0009055 electron carrier activity; GO:0005244 voltage-gated ion channel activity; GO:0022900 electron transport chain; GO:0006954 inflammatory response; GO:0016175 superoxide-generating NADPH oxidase activity; GO:0020037 heme binding; GO:0042554 superoxide anion generation; GO:0043020 NADPH oxidase complex; GO:0045087 innate immune response; View More GO Terms; GO:0045730 respiratory burst; GO:0046982 protein heterodimerization activity; GO:0050660 flavin adenine dinucleotide binding