Cytochrome B245 antibody

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Goat anti Cytochrome B245

Cytochrome B245 antibody recognizes cytochrome b-245 heavy chain, also known as CGD91-phox, cytochrome b(558) subunit beta, cytochrome b558 subunit beta, or p22 phagocyte B-cytochrome. The antibody has been extensively validated for western blotting using whole cell lysates.

Cytochrome B245 Antibody with Control Lysate

Cytochrome B245 antibody recognizes cytochrome b-245 heavy chain, also known as CGD91-phox, cytochrome b(558) subunit beta, cytochrome b558 subunit beta, heme-binding membrane glycoprotein gp91phox, NADPH oxidase 2, or neutrophil cytochrome b 91 kDa polypeptide.

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00132 WB 100 µl
VPA00132KT WB 2 Westerns
With Lysate 400µg
Goat anti Human cytochrome B245 antibody recognizes the cytochrome B245 heavy chain, also known as CGD91-phox, cytochrome b558 subunit beta, heme-binding membrane glycoprotein gp91phox, NADPH oxidase 2, neutrophil cytochrome b 91 kDa polypeptide, superoxide-generating NADPH oxidase heavy chain subunit, gp91-1, gp91-phox and p22 phagocyte B-cytochrome.

Cytochrome B245 is composed of cytochrome B alpha (CYBA) and beta (CYBB) chains. It has been proposed as a primary component of the microbicidal oxidase system of phagocytes. CYBB deficiency is one of five described biochemical defects associated with chronic granulomatous disease (CGD). In this disorder, there is decreased activity of phagocyte NADPH oxidase; neutrophils are able to phagocytize bacteria but cannot kill them in the phagocytic vacuoles. The cause of the killing defect is an inability to increase the cell's respiration and consequent failure to deliver activated oxygen into the phagocytic vacuole (provided by RefSeq, Jul 2008).

Goat anti Human cytochrome B245 antibody detects a band of 80 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti cytochrome B245 detects a band of approximately 80 kDa in HeLa cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Goat polyclonal antibody purified by affinity chromatography.
Preparation
20μl Goat polyclonal antibody purified by affinity chromatography
Buffer Solution
TRIS buffered saline
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
0.02% Sodium Azide (NaN3) 0.5 % BSA
Preservative Stabilisers
0.02% Sodium Azide (NaN3) 0.5 % BSA
Immunogen
Peptide with the sequence C-SYLNFARKRIKNP, from the internal region of the protein sequence.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg HeLa lysate lyophilized in RIPA buffer.
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
P04839 Related reagents
Entrez Gene
CYBB Related reagents
GO Terms
GO:0009055 electron carrier activity
GO:0005244 voltage-gated ion channel activity
GO:0022900 electron transport chain
GO:0006954 inflammatory response
GO:0016175 superoxide-generating NADPH oxidase activity
GO:0020037 heme binding
GO:0042554 superoxide anion generation
GO:0043020 NADPH oxidase complex
GO:0045087 innate immune response
GO:0045730 respiratory burst
GO:0046982 protein heterodimerization activity
GO:0050660 flavin adenine dinucleotide binding
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of Cytochrome B245 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Donkey anti Sheep/Goat IgG:HRP STAR88P 1 ml C E P WB
Donkey anti Sheep/Goat IgG:HRP STAR88P 1 ml C E P WB

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