Cyclin E antibody

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Rabbit anti Cyclin E

Product Type: Polyclonal Antibody
Isotype: Polyclonal IgG

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
AHP1245	E IP WB			50 µl

Rabbit anti Human Cyclin E antibody detects cyclin E, a nuclear protein which is the regulatory subunit of cyclin dependent kinase 2 (Cdk2), and controls S phase entry in cells. Cyclin E follows a cyclic pattern of expression during the cell cycle, with highest expression at the G1-S boundary.

Although cyclin E has an important physiological role in normal cell division, recent reports suggest this function is redundant, and its key functions occur as cells exit quiescence, and in endoreduplication.

Cyclin E is tightly regulated during normal cell cycles, but is often deregulated in carcinomas, lymphoma, and leukaemia.

Product Details

Target Species

Human

Species Cross-Reactivity

Target Species	Cross Reactivity
Mouse
Rat

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Serum - liquid

Antiserum Preparation

Antisera to cyclin E were raised by repeated immunisations of rabbits with highly purified antigen.

Preservative Stabilisers

0.01%

Sodium Azide (NaN₃)

Immunogen

Synthetic peptide corresponding to a region near the C-terminus of human Cyclin E conjugated to KLH.

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.

More Information

UniProt: P24864 Related reagents
Entrez Gene: CCNE1 Related reagents
GO Terms: GO:0000083 regulation of transcription involved in G1/S phase of mitotic cell cycle; GO:0003713 transcription coactivator activity; GO:0005829 cytosol; GO:0005654 nucleoplasm; GO:0030521 androgen receptor signaling pathway; GO:0045893 positive regulation of transcription, DNA-dependent; GO:0050681 androgen receptor binding; GO:0051301 cell division
Regulatory: For research purposes only

Applications of Cyclin E antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
ELISA		1/2000
Immunoprecipitation		1/100
Western Blotting	1/2000	1/10 000

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Western Blotting: AHP1245 detects a band of approximately 45kDa in from human, rat and mouse tissue.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Sheep anti Rabbit IgG:Biotin	2AB02B	1 ml	C E P WB
Goat anti Rabbit IgG (Fc):Biotin	STAR121B	1 mg	E WB
Goat anti Rabbit IgG (Fc):FITC	STAR121F	1 mg	F
Goat anti Rabbit IgG (Fc):HRP	STAR121P	1 mg	E WB
Goat anti Rabbit IgG (H/L):HRP	STAR124P	1 mg	C E WB
Sheep anti Rabbit IgG:FITC	STAR34B	1 mg	C F
Sheep anti Rabbit IgG:RPE	STAR35A	1 ml	F
Sheep anti Rabbit IgG:DyLight®488	STAR36D488GA	0.1 mg	F IF WB
Sheep anti Rabbit IgG:DyLight®549	STAR36D549GA	0.1 mg	F IF
Sheep anti Rabbit IgG:DyLight®649	STAR36D649GA	0.1 mg	F IF
Sheep anti Rabbit IgG:DyLight®680	STAR36D680GA	0.1 mg	F IF WB
Sheep anti Rabbit IgG:Dylight®800	STAR36D800GA	0.1 mg	F IF WB