Cyclin E antibody
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Rabbit anti Human Cyclin E antibody detects cyclin E, a nuclear protein which is the regulatory subunit of cyclin dependent kinase 2 (Cdk2), and controls S phase entry in cells. Cyclin E follows a cyclic pattern of expression during the cell cycle, with highest expression at the G1-S boundary.
Although cyclin E has an important physiological role in normal cell division, recent reports suggest this function is redundant, and its key functions occur as cells exit quiescence, and in endoreduplication.
Cyclin E is tightly regulated during normal cell cycles, but is often deregulated in carcinomas, lymphoma, and leukaemia.
Although cyclin E has an important physiological role in normal cell division, recent reports suggest this function is redundant, and its key functions occur as cells exit quiescence, and in endoreduplication.
Cyclin E is tightly regulated during normal cell cycles, but is often deregulated in carcinomas, lymphoma, and leukaemia.
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Serum - liquid
- Antiserum Preparation
- Antisera to cyclin E were raised by repeated immunisations of rabbits with highly purified antigen.
- Preservative Stabilisers
0.01% Sodium Azide (NaN3) - Immunogen
- Synthetic peptide corresponding to a region near the C-terminus of human Cyclin E conjugated to KLH.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P24864
- Entrez Gene
- CCNE1
- GO Terms
- GO:0000083 regulation of transcription involved in G1/S phase of mitotic cell cycle
- GO:0003713 transcription coactivator activity
- GO:0005829 cytosol
- GO:0005654 nucleoplasm
- GO:0030521 androgen receptor signaling pathway
- GO:0045893 positive regulation of transcription, DNA-dependent
- GO:0050681 androgen receptor binding
- GO:0051301 cell division
- Regulatory
- For research purposes only
Applications of Cyclin E antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 1/2000 | ||
| Immunoprecipitation | 1/100 | ||
| Western Blotting | 1/2000 | 1/10 000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- AHP1245 detects a band of approximately 45kDa in from human, rat and mouse tissue.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
|
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| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
|
|
| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
|
|
| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
|
|
| Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
|
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| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
|
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| Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | F IF WB | 0.1 mg |
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml |
Product Specific References
Further Reading
-
Hwang, H.C. & Clurman, B.E. (2005) Cyclin E in normal and neoplastic cell cycles.
Oncogene. 24 (17): 2776-86. -
Zhang, H. (2007) Life without kinase: cyclin E promotes DNA replication licensing and beyond.
Mol Cell. 25 (2): 175-6.
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