Cyclin E antibody

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Rabbit anti Cyclin E

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP1245 E IP WB 50 µl
Rabbit anti Human Cyclin E antibody detects cyclin E, a nuclear protein which is the regulatory subunit of cyclin dependent kinase 2 (Cdk2), and controls S phase entry in cells. Cyclin E follows a cyclic pattern of expression during the cell cycle, with highest expression at the G1-S boundary.

Although cyclin E has an important physiological role in normal cell division, recent reports suggest this function is redundant, and its key functions occur as cells exit quiescence, and in endoreduplication.

Cyclin E is tightly regulated during normal cell cycles, but is often deregulated in carcinomas, lymphoma, and leukaemia.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Serum - liquid
Antiserum Preparation
Antisera to cyclin E were raised by repeated immunisations of rabbits with highly purified antigen.
Preservative Stabilisers
0.01%Sodium Azide (NaN3)
Immunogen
Synthetic peptide corresponding to a region near the C-terminus of human Cyclin E conjugated to KLH.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P24864 Related reagents
Entrez Gene
CCNE1 Related reagents
GO Terms
GO:0000083 regulation of transcription involved in G1/S phase of mitotic cell cycle
GO:0003713 transcription coactivator activity
GO:0005829 cytosol
GO:0005654 nucleoplasm
GO:0030521 androgen receptor signaling pathway
GO:0045893 positive regulation of transcription, DNA-dependent
GO:0050681 androgen receptor binding
GO:0051301 cell division
Regulatory
For research purposes only

Applications of Cyclin E antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/2000
Immunoprecipitation 1/100
Western Blotting 1/2000 1/10 000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1245 detects a band of approximately 45kDa in from human, rat and mouse tissue.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
TidyBlot™ Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*

Product Specific References

Further Reading

  1. Hwang, H.C. & Clurman, B.E. (2005) Cyclin E in normal and neoplastic cell cycles.
    Oncogene. 24 (17): 2776-86.
  2. Zhang, H. (2007) Life without kinase: cyclin E promotes DNA replication licensing and beyond.
    Mol Cell. 25 (2): 175-6.
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