Rabbit anti Human CXCL9 polyclonal antinbody
recognizes human CXCL9, otherwise known as MIG (monokine induced by Interferon-gamma), a member of the CXC family of chemokine receptors, secreted by interferon stimulated monocytes, macrophages (Muller et al. 2010
) and endothelial cells (Loos et al. 2006
) CXCL9 signals through binding to CD183
CXCL9 acts as a Th1 (type 1 helper T) cell chemoattractant, and plays a role in the growth, activation and movement of cells associated with immune and inflammatory responses
, and in tumour growth inhibition and angiogenesis (Whiting et al. 2004
) . Studies have shown that CXCL9 is active against E.coli, S.aureus, L.monocytogenes
(Cole et al. 2001
) and S.pyogenes
(Egesten et al. 2007
- Target Species
- Product Form
- Purified IgG - lyophilised
- Reconstitute with 0.1ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
- Antiserum Preparation
- Antiserum to human CXCL9 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Carrier Free
- Recombinant human CXCL9
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml after reconstitution
- Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 6 months from date of despatch
- Entrez Gene
- GO Terms
cellular defense response
G-protein coupled receptor protein signaling pathway
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of CXCL9 antibody
|Immunohistology - Paraffin 1
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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Secondary Antibodies Available
Useful Reagents Available
Product Specific References
References for CXCL9 antibody
Ciccia, F. et al. (2014) Macrophage phenotype in the subclinical gut inflammation of patients with ankylosing spondylitis.
Rheumatology (Oxford). 53 (1): 104-13.
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