CXCL9 antibody

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Anti Human CXCL9 Antibody thumbnail image 2

Anti Human CXCL9 Antibody gallery image 1 — Formalin fixed, paraffin embedded human renal tumor with parenchymal tissue stained with Rabbit anti Human CXCL9 (**AHP1864**) following heat mediated antigen retrieval using sodium citrate buffer (pH6.0)

Anti Human CXCL9 Antibody gallery image 2 — Formalin fixed, paraffin embedded human renal tumor with parenchymal tissue stained with Rabbit anti Human CXCL9 (**AHP1864**) following heat mediated antigen retrieval using sodium citrate buffer (pH6.0)

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Rabbit anti Human CXCL9

Product Type: Polyclonal Antibody
Isotype: Polyclonal IgG
Specificity: CXCL9

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Secondary Antibodies
References (1)
Applications
Useful Reagents
Storage Information
Batch/Lot Specific Datasheets

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Product Code Applications Pack Size List Price Your Price Qty

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AHP1864 Datasheet Datasheet SDS SDS	E FN P * WB	0.1 mg		Log in	Get Quote
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Rabbit anti Human CXCL9 polyclonal antinbody recognizes human CXCL9, otherwise known as MIG (monokine induced by Interferon-gamma), a member of the CXC family of chemokine receptors, secreted by interferon stimulated monocytes, macrophages (Muller et al. 2010) and endothelial cells (Loos et al. 2006). CXCL9 signals through binding to CD183 (CXCR3).

CXCL9 acts as a Th1 (type 1 helper T) cell chemoattractant, and plays a role in the growth, activation and movement of cells associated with immune and inflammatory responses, and in tumou growth inhibition and angiogenesis (Whiting et al. 2004) . CXCL9 is active against E.coli, S.aureus, L.monocytogenes (Cole et al. 2001) and S.pyogenes (Egesten et al. 2007).

Target Species: Human
Product Form: Purified IgG - lyophilized
Reconstitution: Reconstitute with 0.1ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
Antiserum Preparation: Antiserum to human CXCL9 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: None present
Carrier Free: Yes
Immunogen: Recombinant human CXCL9
Approx. Protein Concentrations: IgG concentration 1.0mg/ml after reconstitution
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch

Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.

This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
ELISA	0.5	2.0ug/ml
Functional Assays	5.0	7.0ug/ml
Immunohistology - Paraffin ¹
Western Blotting	0.1	0.2ug/ml

¹This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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References for CXCL9 antibody

Ciccia, F. et al. (2014) Macrophage phenotype in the subclinical gut inflammation of patients with ankylosing spondylitis.
Rheumatology (Oxford). 53 (1): 104-13.

Synonyms: MIG
RRID: AB_2086883
UniProt: Q07325
Entrez Gene: CXCL9
GO Terms: GO:0005615 extracellular space; GO:0006935 chemotaxis; GO:0006954 inflammatory response; GO:0006955 immune response; GO:0006968 cellular defense response; GO:0007186 G-protein coupled receptor protein signaling pathway; GO:0007267 cell-cell signaling; GO:0008009 chemokine activity