Cxadr antibody

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Rabbit anti Cxadr

CXADR antibody recognizes the coxsackievirus and adenovirus receptor, also known as 46 kD coxsackievirus and adenovirus receptor (CAR) protein or HCAR.

Cxadr Antibody with Control Lysate

CXADR antibody recognizes the coxsackievirus and adenovirus receptor, also known as 46 kD coxsackievirus and adenovirus receptor (CAR) protein or HCAR.

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00832 WB 100 µl
VPA00832KT WB 2 Westerns
With Lysate 400µg
Rabbit anti Human CXADR antibody recognizes the coxsackievirus and adenovirus receptor, also known as 46 kD coxsackievirus and adenovirus receptor (CAR) protein, HCAR, CVB3-binding protein, HCVADR and coxsackievirus B-adenovirus receptor.

The protein encoded by CXADR is a type I membrane receptor for group B coxsackieviruses and subgroup C adenoviruses. Several transcript variants encoding different isoforms have been found for CXADR. Pseudogenes of CXADR are found on chromosomes 15, 18, and 21 (provided by RefSeq, May 2011).

Rabbit anti Human CXADR antibody detects bands of 45-55 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti CXADR antibody recognizes a band of approximately 45-55 kDa in HeLa cell lysates
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography on immunogen
Preparation
20μl Rabbit polyclonal antibody purified by affinity chromatography on immunogen
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
<50% Glycerol
Preservative Stabilisers
0.09% Sodium Azide
<50% Glycerol
Immunogen
Recombinant protein of human CXADR
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg HeLa lysate lyophilized in RIPA buffer
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20°C, avoiding repeated freeze thaw cycles
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
P78310 Related reagents
Entrez Gene
CXADR Related reagents
GO Terms
GO:0007596 blood coagulation
GO:0007155 cell adhesion
GO:0005576 extracellular region
GO:0004872 receptor activity
GO:0005887 integral to plasma membrane
GO:0005634 nucleus
GO:0005737 cytoplasm
GO:0005912 adherens junction
GO:0005923 tight junction
GO:0016323 basolateral plasma membrane
GO:0044419 interspecies interaction between organisms
GO:0050776 regulation of immune response
GO:0050900 leukocyte migration
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of Cxadr antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB

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